Rao / Lavagi-Craddock / Vidalakis Viroids

Methods and Protocols
Erscheinungsjahr 2021
ISBN: 978-1-0716-1464-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2316, 375 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rao, Ayala L. N.

Lavagi-Craddock, Irene

Vidalakis, Georgios

Inhaltsverzeichnis

Study and Detection of Citrus Viroids in Woody Hosts.- Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts.- Analysis on RNA Motif-Based RNA Trafficking in Plants.- Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis.- High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids.- Extraction and Purification of Viroids from Herbaceous Hosts.- Detection of Viroids by sPAGE Gel Electrophoresis.- Two-Dimensional Polyacrylamide-Gel Electrophoresis Analysis of Viroid RNAs.- A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae.- Gel Blot Hybridization for Viroids.- Tissue Print Hybridization for Detection and Characterization of Viroids.- Fluorescein-Based Electrophoretic Mobility Shift Assay.- Detection of Viroids by RT-PCR.- Detection of Viroids using RT-qPCR.- Multiplex RT-PCR.- Real-TimeDetection of Viroids using Singleplex and Multiplex Quantitative Polymerase Chain Reaction.- Reverse Transcription-Loop Mediated Isothermal AMPlification (RT-LAMP) at the Service of Viroid Detection.- SYBR ® Green RT-qPCR for the Universal Detection of Citrus Viroids.- Detection of Avocado sunblotch and Other Viroids using RNA Filter Paper Capture and RT-PCR.- Cloning and Sequencing of Viroids.- QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification.- Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms.- An In Silico Detection of a Citrus Viroid from Raw High Throughput Sequencing Data.- Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs.- Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold.- Predicting the Structure of a Viroid.