Rapley / Walker Molecular Biomethods Handbook

1998
ISBN: 978-1-59259-642-3
Verlag: Humana Press
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 738 Seiten, eBook

Reihe: Springer Protocols Handbooks

An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Rapley, Ralph

Walker, John M.

Inhaltsverzeichnis

Extraction of Total RNA from Tissues and Cultured Cells.- Isolation of Total Cellular DNA from Tissues and Cultured Cells.- Gel Electrophoresis of DNA.- Sl Nuclease Analysis of RNA.- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA).- Gene Probes.- Nonradioactive Labeling of DNA.- Southern Blot Analysis.- Northern Blot Analysis.- DNA Sequencing.- Autoradiography and Fluorography.- Mobility Shift Assays.- cDNA Libraries.- Genomic DNA Libraries.- ? as a Cloning Vector.- Plasmid-Derived Cloning Vectors.- M13 and Phagemid-Based Cloning Vectors.- Retroviral Vectors From Laboratory Tools to Molecular Medicines.- Baculovirus Vectors.- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes.- Plant Transformation.- Restriction Fragment-Length Polymorphisms.- Genome Mapping.- Yeast Artificial Chromosomes.- Polymerase Chain Reaction.- In Vitro Transcriptions.- In Vitro Translation.- Site-Directed Mutagenesis.- Site-Directed Mutagenesis.- DNA Profiling.- Radiolabeling of Peptides and Proteins.- Protein Electrophoresis.- Free Zone Capillary Electrophoresis.- Protein Blotting.- Ion-Exchange Chromatography.- Size-Exclusion Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- Reversed-Phase HPLC.- Glycoprotein Analysis.- Protein Sequencing.- Solid-Phase Peptide Synthesis.- Protein Engineering.- Monoclonal Antibodies.- Phage-Display Libraries.- Enzyme- Linked Immunosorbent Assay (ELISA).- Epitope Mapping.- lmmunocytochemistry.- Flow Cytometry.- Mass Spectrometry.- The Technique of In Situ Hybridization.