Rickwood Biochemistry LabFax

1. Auflage 2014
ISBN: 978-1-4832-9444-5
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 361 Seiten, Web PDF

LABFAX volumes are purpose-designed data reference books for practising scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogues since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs.Biochemistry LabFax is a detailed compendium of essential information - on radioisotopes, enzymes, proteins, nucleic acids, lipids, plus data on selected techniques such as chromatography, electrophoresis, etc. - needed on an almost daily basis by researchers in any area of biochemistry. In addition, the book acts as a gateway to other sources for information more specific than can be covered in a volume of this size.

Autoren/Hrsg.

Rickwood, D.

Inhaltsverzeichnis

1;Front Cover;1
2;Biochemistry Labfax;4
3;Copyright Page;5
4;Table of Contents;8
5;PREFACE;6
6;HAZARD WARNING;7
7;CONTRIBUTORS;16
8;ABBREVIATIONS;18
9;CHAPTER 1.
BUFFERS, CHELATING AGENTS AND DENATURANTS;22
9.1;1. BUFFERS;22
9.2;2. CHELATING AGENTS;32
9.3;3. DENATURING AGENTS FOR PROTEINS;34
9.4;4.
THIOL REAGENTS;38
9.5;5. DETERGENTS;38
9.6;6. PROPERTIES OF ACIDS, BASES, SALTS AND ORGANIC SOLVENTS;43
9.7;7. AMINO ACIDS;43
9.8;8. AMMONIUM SULFATE PRECIPITATION CHART FOR PROTEINS;54
9.9;9. REFERENCES;56
10;CHAPTER 2.
RADIOISOTOPES IN BIOCHEMISTRY;58
10.1;1. DEFINITIONS;58
10.2;2. RADIOISOTOPES USED IN BIOCHEMISTRY;58
10.3;3. RADIOACTIVE DECAY CORRECTION;60
10.4;4. AUTORADIOGRAPHY;60
10.5;5. SCINTILLATION COUNTING;62
10.6;6. USE OF RADIOISOTOPES AS TRACERS;64
10.7;7. RADIOLOGICAL PROTECTION;66
10.8;8. METHODS
FOR DECONTAMINATING LABORATORIES;67
10.9;9. REFERENCES;69
11;CHAPTER 3.
CHROMATOGRAPHIC FRACTIONATION MEDIA;70
11.1;1. INTRODUCTION;70
11.2;2. MEDIA FOR SIZE EXCLUSION;71
11.3;3. MEDIA FOR ION-EXCHANGE SEPARATIONS;71
11.4;4. MEDIA FOR REVERSED-PHASE SEPARATIONS;71
11.5;5. MEDIA FOR AFFINITY CHROMATOGRAPHY;71
11.6;7. REFERENCE;89
11.7;8. FURTHER READING;89
12;CHAPTER 4. ELECTROPHORESIS OF PROTEINS AND NUCLEIC ACIDS;90
12.1;1. PROTEINS;90
12.2;2. NUCLEIC ACIDS;95
12.3;3. METHODS MANUALS ON ELECTROPHORESIS;105
13;CHAPTER 5.
GENERAL CENTRIFUGATION DATA;106
13.1;1. CALCULATION OF CENTRIFUGAL FORCE;106
13.2;2. APPLICATIONS OF CENTRIFUGE ROTORS;106
13.3;3. CALCUUTION OF k-FACTORS OF ROTORS AND PELLETING
TIMES;106
13.4;4. DERATING ROTORS FOR USE WITH DENSE SOLUTIONS;107
13.5;5. PROPERTIES OF CENTRIFUGE TUBES AND BOHLES;107
13.6;6. STERILIZATION AND DISINFECTION PROCEDURES;117
13.7;7. EQUATIONS RELATING THE REFRACTIVE INDEX TO THE DENSITY OF SOLUTIO;119
13.8;8. PROPERTIES OF SUCROSE SOLUTIONS;120
14;CHAPTER 6.
ENZYMOLOGY;122
14.1;1. INTRODUCTION;122
14.2;2. ENZYME KINETICS;122
14.3;3. ENZYME INHIBITORS;136
14.4;4. EFFECTS OF pH ON ENZYMES;151
14.5;5. COENZYMES
– STRUCTURE AND FUNCTIONS;151
14.6;6. ENZYME ASSAYS;151
14.7;7. HANDLING AND STORAGE OF ENZYMES AND COENZYMES;162
14.8;8. REFERENC;163
14.9;9. FURTHER READING;164
15;CHAPTER 7. HYDROLYTIC ENZYMES;166
15.1;1. INTRODUCTION;166
15.2;2. INHIBITION OF NUCLEASES AND PROTEASES;166
15.3;3. NATURALLY OCCURRING OR PHYSIOLOGICAL INHIBITORS;168
15.4;4. REFERENCES;185
16;CHAPTER 8.
CHARACTERISTICS OF SELECTED PROTEINS;188
16.1;REFERENCES;211
17;CHAPTER 9. GLYCOPROTEINS AND PROTEIN GLYCOSYLATION;214
17.1;1. INTRODUCTION;214
17.2;2. OCCURRENCE OF GLYCOSYLATED PROTEINS IN NATURE;214
17.3;3. STRUCTURAL FEATURES OF GLYCOSYLATED PROTEINS;217
17.4;4. ENZYMIC GLYCOSYLATION
– SYNTHESIS OF SEQUENCE;224
17.5;5. EXAMPLES OF FUNCTIONAL ROLES FOR PROTEIN GLYCOSYLATION;232
17.6;6. REFERENCES;235
18;CHAPTER 10. CHEMICAL AND POST-TRANSLATIONAL MODIFICATION OF PROTEINS;236
18.1;1. CHEMICAL MODIFICATION OF PROTEINS;236
18.2;2. ENZYME-CATALYZED COVALENT MODIFICATION REACTIONS: AN OVERVIEW;236
18.3;3. REFERENCES;260
19;CHAPTER 11.
NUCLEIC ACIDS AND THEIR COMPONENTS;268
19.1;1. NUCLEOSIDES AND NUCLEOTIDES;268
19.2;2. DNA OF SELECTED ORGANISMS;272
19.3;3. THE GENETIC CODE;272
19.4;4. ABBREVIATIONS OF AMINO ACIDS;282
19.5;5. ASSAYS FOR NUCLEIC ACIDS;283
19.6;6. REFERENCES;283
20;CHAPTER 12.
LIPIDS;288
20.1;1. INTRODUCTION;288
20.2;2. STRUCTURE AND CHARACTERISTICS OF ACYL LIPIDS;288
20.3;3. STRUCTURE AND DISTRIBUTION OF THE MAJOR TERPENOID CONSTITUENTS OF
MEMBRANES;299
20.4;4. COMPOSITION AND DISTRIBUTION OF LIPIDS IN MEMBRANES;303
20.5;5. STRUCTURE AND PROPERTIES OF BIOACTIVE LIPIDS;309
20.6;6. STRUCTURE AND COMPOSITION OF BILE ACIDS AND BILE SALTS;321
20.7;7. CHEMICAL ABSTRACTS REGISTRY NUMBERS OF LIPIDS CITED;322
20.8;8. REFERENCES;322
21;CHAPTER 13.
CARBOHYDRATES AND SUGARS;326
21.1;1. INTRODUCTION;326
21.2;2. STRUCTURES AND CHARACTERISTICS
OF MONOMERIC CARBOHYDRATES;326
21.3;3. OLIGOSACCHARIDES;331
21.4;4. STRUCTURES AND CHARACTERISTICS OF POLYSACCHARIDES;333
21.5;5. FURTHER READING;336
22;CHAPTER 14. SAFETY AND THE DISPOSAL OF TOXIC AND
INFECTIOUS MATERIALS;338
22.1;1. PROTECTION FROM CHEMICAL HAZARDS;338
22.2;2. DISPOSAL OF TOXIC AND INFECTIOUS MATERIALS;343
22.3;3. REFERENCES;351
23;CHAPTER 15.
SOURCES OF FURTHER BIOCHEMICAL DATA;352
23.1;1. REFERENCE BOOKS;352
23.2;2. DATABASES;352
23.3;3. NUCLEIC ACID SEQUENCE DATABASES;363
23.4;4. CONTACT ADDRESSES;364
24;CHAPTER 16. ATOMIC WEIGHTS AND MATHEMATICAL FORMULAE;366
24.1;1. ATOMIC WEIGHTS;366
24.2;2. MATHEMATICAL FORMULAE;368
25;INDEX;378