Sagi / Afratis Collagen

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9095-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1944, 254 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sagi, Irit

Afratis, Nikolaos A.

Inhaltsverzeichnis

Cloning and Mutagenesis Strategies for Large Collagens.- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen.- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds.- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils.- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy.- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry.- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes.- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics.- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy.- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology.- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics.- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix.- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans.- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis.- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment.- FACS Analysis of Col1a Protein Levels in Primary Fibroblasts.- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors.