Single-Channel Recording

2. Auflage 1995
ISBN: 978-0-306-44870-6
Verlag: Springer Us

Buch, Englisch, 700 Seiten, Format (B × H): 183 mm x 259 mm, Gewicht: 1451 g

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

Zielgruppe

Research

Autoren/Hrsg.

Sakmann, Bert

Neher, Erwin

Fachgebiete

• Sozialwissenschaften Psychologie Allgemeine Psychologie Biologische Psychologie, Neuropsychologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierphysiologie

Inhaltsverzeichnis

to Patch Clamping.- A Practical Guide to Patch Clamping.- Tight-Seal Whole-Cell Recording.- Guide to Data Acquisition and Analysis.- Methods.- Electronic Design of the Patch Clamp.- Low-Noise Recording.- Voltage Offsets in Patch-Clamp Experiments.- Techniques for Membrane Capacitance Measurements.- Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices.- Patch Clamp and Calcium Imaging in Brain Slices.- Fast Application of Agonists to Isolated Membrane Patches.- Electrochemical Detection of Secretion from Single Cells.- Technical Approaches to Studying Specific Properties of Ion Channels in Plants.- The Giant Membrane Patch.- A Fast Pressure-Clamp Technique for Studying Mechanogated Channels.- Electrophysiological Recordings from Xenopus Oocytes.- Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.- Force Microscopy on Membrane Patches.- Analysis.- The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- Fitting and Statistical Analysis of Single-Channel Records.- A Q-Matrix Cookbook.- “Classics”.- Geometric Parameters of Pipettes and Membrane Patches.- Conformational Transitions of Ionic Channels.