Sakmann / Neher Single-Channel Recording

2. Auflage 1995
ISBN: 978-1-4419-1229-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 700 Seiten, eBook

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

Zielgruppe

Research

Autoren/Hrsg.

Sakmann, Bert

Neher, Erwin

Inhaltsverzeichnis

to Patch Clamping.- A Practical Guide to Patch Clamping.- Tight-Seal Whole-Cell Recording.- Guide to Data Acquisition and Analysis.- Methods.- Electronic Design of the Patch Clamp.- Low-Noise Recording.- Voltage Offsets in Patch-Clamp Experiments.- Techniques for Membrane Capacitance Measurements.- Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices.- Patch Clamp and Calcium Imaging in Brain Slices.- Fast Application of Agonists to Isolated Membrane Patches.- Electrochemical Detection of Secretion from Single Cells.- Technical Approaches to Studying Specific Properties of Ion Channels in Plants.- The Giant Membrane Patch.- A Fast Pressure-Clamp Technique for Studying Mechanogated Channels.- Electrophysiological Recordings from Xenopus Oocytes.- Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.- Force Microscopy on Membrane Patches.- Analysis.- The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- Fitting and Statistical Analysis of Single-Channel Records.- A Q-Matrix Cookbook.- “Classics”.- Geometric Parameters of Pipettes and Membrane Patches.- Conformational Transitions of Ionic Channels.