Sasano / Suzuki Interface Oral Health Science 2009

Proceedings of the 3rd International Symposium for Interface Oral Health Science, Held in Sendai, Japan, Between January 15 and 16, 2009 and the 1st Tohoku-Forsyth Symposium, Held in Boston, MA, USA, Between March 10 and 11, 2009
1. Auflage 2010
ISBN: 978-4-431-99644-6
Verlag: Springer Japan
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 435 Seiten

Since 2002, the Tohoku University Graduate School of Dentistry has proposed 'Interface Oral Health Science' as a major theme for next-generation dental research. That theme is based on the following new concept: healthy oral fu- tion is maintained by biological and biomechanical harmony among three s- tems: (1) oral tissues (host); (2) parasitic microorganisms of the oral cavity (parasites); and (3) biomaterials. The concept implies that oral diseases such as dental caries, periodontal disease, and temporomandibular disorders should be interpreted as 'interface disorders' that result from disruption of the intact int- face among these systems. The uniqueness of this concept rests on the fact that it not only encompasses the field of dentistry and dental medicine, but also expands the common ground shared with other fields, including medicine, ag- culture, material science, engineering, and pharmacology. We aim to promote advances in dental research and to activate collaboration with related fields by putting interface oral health science into practice. On this basis, we have already organized the 1st and 2nd International Symposiums for Interface Oral Health Science, which included inspiring special lectures, symposiums, poster pres- tations, and other discussions. The contents of the two symposiums were p- lished as monographs entitled Interface Oral Health Science in 2005 and 2007. The 3rd International Symposium was held in January 2009 as part of this project.

Autoren/Hrsg.

Sasano, Takashi

Suzuki, Osamu

Inhaltsverzeichnis

1;Preface;5
2;Commentary to The 1st Tohoku-Forsyth Symposium;7
3;Contents;9
4;Shear-stress-sensing and response mechanisms in vascular endothelial cells;23
4.1;1 Introduction;23
4.2;2 Ca.2+. Signaling of Shear Stress;24
4.3;3 P2X4 Channels Mediate Ca.2+. Influx in Response to Shear Stress;24
4.4;4 Shear-Stress-Induced ATP Release Via Cell-Surface ATP Synthase;26
4.5;5 Vascular Physiological Roles of Ca.2+. Signaling of Shear Stress;26
4.6;References;28
5;Cleft formation and branching morphogenesis of salivary gland: exploration of new functional genes;31
5.1;1 Introduction;31
5.2;2 Branching Morphogenesis: Embryonic Submandibular Gland Development;33
5.3;3 A Strategy to Characterize Molecules Necessary for Branching Morphogenesis;33
5.4;4 Laser Microdissection;33
5.5;5 T7-SAGE;33
5.6;6 The Essential Role of Fibronectin in Branching Morphogenesis;34
5.7;7 Other ECM Molecules;35
5.8;8 Conclusions;36
5.9;References;36
6;Strategies underlying research in tooth regenerative therapy as a possible model for future organ replacement;38
6.1;1 Introduction;38
6.2;2 The Strategies Underlying Current Research on Tooth Regenerative Therapy;39
6.3;3 The Development of a Novel Bioengineered Organ Germ Method;39
6.4;4 Eruption and Structure af a Bioengineered Tooth;40
6.5;5 Functional Bioengineered Tooth Replacement In an Adult Oral Environment;41
6.6;6 Conclusions;43
6.7;References;43
7;Molecular basis for specification of the vertebrate head field;45
7.1;1 Introduction;45
7.2;Axis Formation by the Dorsal Organizer;46
7.3;3 Region-Specific Induction by the Dorsal Organizer;46
7.4;4 Specification of the Head in Mice;46
7.5;5 Role of Head Organizer Genes in Head Formation;48
7.6;6 Role of FGF Signal Inhibitor in Head Formation;49
7.7;References;49
8;Dental epithelium proliferation and differentiation regulated by ameloblastin;51
8.1;1 Ameloblast Differentiation;51
8.2;2 Amelogenesis Imperfecta Resulting from Mutation in Enamel Matrix;53
8.3;3 Expression of Enamel Matrix During Tooth Development;53
8.4;4 Structure of Ameloblastin and Regulatory Mechanism of Proliferation by Ameloblastin;54
8.5;References;56
9;Stress fiber and the mechanical states in a living endothelial cell;58
9.1;1 Introduction;58
9.2;2 Materials and Methods;59
9.2.1;2.1 Cell Culture;59
9.2.2;2.2 Isolation of Stress Fiber;59
9.2.3;2.3 Evaluation of Preexisting Strain of Stress Fiber;60
9.2.4;2.4 Tensile Test of Stress Fiber;60
9.3;3 Results and Discussion;61
9.4;References;63
10;Transient receptor potential channels and mechanobiology;65
10.1;1 Classification of Ionic Channels;66
10.2;2 Mechanosensitive Channels;66
10.3;3 Mechanosensitive Channels;67
10.4;References;68
11;Molecular mechanisms of the response to mechanical stimulation during chondrocyte differentiation;70
11.1;1 Introduction;71
11.2;2 General Mechanoreaction of Cells;71
11.3;3 Differentiation of Chondrocytes;72
11.4;4 Mechanobiology of Chondrocytes;73
11.4.1;4.1 Mechanoresponse of Chondrocytes;73
11.4.2;4.2 Mechanotransduction in Chondrocytes;74
11.5;5 Summary;75
11.6;References;75
12;Recruitment of masseter motoneurons by spindle Ia inputs and its modulation by leak K.+. channels;77
12.1;1 Recruitment of Masseter Motoneurons Caused by Ia Inputs During the Slow-Closing Phase;77
12.2;2 Possible involvements of leak K.+. channels, TASK1/3, in IR-ordered recruitment;80
12.3;References;82
13;Implant interface to bone tissue: biomimetic surface functionalization through nanotechnology;84
13.1;Introduction;84
13.2;2 Bone: A Hybrid Structure of Organic Matrix and Crystalline Hydroxyapatite;85
13.3;3 Biomimetic Surface Functionalization;86
13.4;Conclusions;87
13.5;References;89
14;Interface affinity between apatites and biological tissues;91
14.1;1 Introduction;91
14.2;2 New Concept for Biological Adhesion;92
14.3;3 Effect of Mg.2+. Ions on Bone Formation;92
14.4;4 BMP Modification;94
14.5;5 SVVYGLR Modification;95
14.6;6 Summary;96
14.7;References;96
15;Biological reactions on titanium surface electrodeposited biofunctional molecules;98
15.1;Introduction;98
15.2;Immobilization of PEG to metals with electrodeposition;99
15.3;Immobilization of biomolecules;102
15.4;Conclusions;103
15.5;References;103
16;Effect of Young’s modulus in metallic implants on atrophy and bone remodeling;105
16.1;1 Introduction;105
16.2;2 Lowering Young’s Modulus of Titanium Alloy Similar to That of Cortical Bone;106
16.3;3 Development of Mechanical Endurance of Biomedical Titanium Alloy with Keeping Young’s Modulus Low;109
16.4;4 Young’s Modulus and Bone Atrophy;110
16.5;5 Young’s Modulus and Bone Remodeling;113
16.6;6 Summary;114
16.7;References;114
17;Chemical and physical factors affecting osteoconductivity of octacalcium phosphate bone substitute material;115
17.1;1 Introduction;115
17.2;2 Activation of Osteoblasts and Osteoclasts by OCP;116
17.3;3 Effect of Stoichiometry of OCP on Osteoconductivity;117
17.4;4 OCP–HA Conversion;118
17.5;5 Effect of Mechanical Stress on Osteoconductivity of OCP;120
17.6;6 Summary;121
17.7;References;121
18;Effects of zebularine on the apoptosis of 5-fluorouracil via cAMP/PKA/CREB pathway in HSC-3 cells;124
18.1;1 Introduction;124
18.2;2 Results and Conclusions;125
18.3;Reference;125
19;Wnt signaling inhibits cementoblast differentiation;126
19.1;1 Introduction;126
19.2;2 Results and Discussion;128
19.3;References;128
20;Prevention of necrotic actions of nitrogen-containing bisphosphonates (NBPs) in mice by non-NBPs (clodronate and etidronate);129
20.1;1 Introduction;130
20.2;2 Materials and Method;130
20.3;3 Summary of Results;130
20.4;4 Discussion;131
21;Interface, implant, regenerated bone and recipient alveolar bone;132
21.1;1 Introduction;133
21.2;2 Material and Methods;133
21.3;3 Results and Discussion;134
21.4;4 Conclusions;135
21.5;References;135
22;Activation of matrix metalloproteinase-2 at the interface between epithelial cells and fibroblasts from human periodontal li;136
22.1;Introduction;136
22.2;Materials and methods;137
22.3;Results;137
22.4;Conclusion;137
22.5;References;138
23;Histomorphometric study of alveolar bone-implant (miniscrew) interface used as an orthodontic anchorage;139
23.1;Introduction;139
23.2;Histomorphometric Indices of Alveolar Bone Surrounding Miniscrew;140
23.3;Cortical Bone Thickness Surrounding Miniscrew;140
23.4;Conclusion;140
24;Mechanical stress modulates bone remodeling signals;142
24.1;1 Introduction;143
24.2;2 Materials and Methods;143
24.2.1;2.1 Cell Culture;143
24.2.2;2.2 Mechanical Stretch Loading;143
24.2.3;2.3 RNA Isolation and DNA Microarray;143
24.2.4;2.4 Semiquantitative RT-PCR Amplification;144
24.3;3 Results and Discussion;144
24.4;References;145
25;Expression analysis of p51/p63 in enamel organ epithelial cells;146
25.1;1 Introduction;146
25.2;2 Expression Pattern of p51 in emtg-1 to -5 Cells;147
25.3;3 Effects of p51 Knockdown on emtg-2 Cells;147
25.4;Functions of p51 in Tooth Development;147
25.5;References;148
26;Osteogenesis by gradually expanding the interface between bone surface and periosteum: preliminary analysis of the use of nov;149
26.1;References;150
27;Possible role of Ccn family members during osteoblast differentiation;151
27.1;References;152
28;Inhibition of oral fibroblast growth and function by N-acetyl cysteine;153
28.1;1 Introduction;153
28.2;2 Effects of NAC on Growth and Function of Oral Fibroblasts;154
28.3;Discussion;155
28.4;References;155
29;Computer simulation of orthodontic tooth movement using FE analysis;156
30;Mechanical-stress-induced apoptosis and angiogenesis in periodontal tissue;158
30.1;1 Introduction;158
30.2;2 Osteoblast Apoptosis by Continuous/Compressive Force;159
30.3;3 Continuous Compressive Force Increased the Expression of Vascular Endothelial Growth Factor in PDL Cells;159
30.4;4 Conclusion;160
30.5;References;160
31;Diachronic changes of tooth wear in the deciduous dentition of the Japanese;161
31.1;1 Introduction;161
31.2;2 Materials and Methods;161
31.3;3 Results and Discussion;162
31.4;References;162
32;Dental occlusal deformation analysis of porcine mandibular periodontium using digital image correlation method;163
32.1;Introduction;163
32.2;2 Summary;164
33;Measurement of the transmitted-light through human upper incisors;166
33.1;1 Introduction;166
33.2;2 Materials and Methods;167
33.3;3 Results;167
33.4;4 Discussion and Conclusion;167
33.5;References;167
34;Three-dimensional finite element analysis of overload-induced alveolar bone resorption around dental implants;168
34.1;1 Introduction;168
34.2;2 Materials and Methods;169
34.3;3 Results and Conclusions;169
34.4;Reference;170
35;Regulation of microrna expression by bone morphogenetic protein-2;171
35.1;1 Regulation of miRNA Expression by Bone Morphogenetic Protein (Bmp)-2;171
35.2;2 Regulation of the miRNA Processing Pathway by BMP-2;172
35.3;References;172
36;Influence of early progressive loading on implants placed into extraction sockets;174
37;In vitro gene transfection of human stromal cell derived factor-1.a. and its expression in rat myoblasts;176
38;Biomarker identification in oral cancer by using proteomics;180
38.1;1 Introduction;180
38.2;References;181
39;In vivo analysis of the 3-D force on implants supporting fixed prostheses;182
39.1;1 Introduction;182
39.2;2 Measurement of 3-D Load on Implants;183
39.3;3 Effects of Splinting the Superstructures on the Loads;183
39.4;4 Clinical Implication;183
39.5;5 Conclusions;184
39.6;References;184
40;Gap junctional communication regulates salivary gland morphogenesis;185
40.1;1 Introduction;185
40.2;2 Materials and Methods;186
40.3;Results and Discussion;186
40.4;References;186
41;Pulpal blood flow in human permanent teeth with different root formation;187
41.1;1 Introduction;187
41.2;2 Materials and Methods;188
41.3;3 Results;188
41.4;4 Discussion and Conclusion;188
41.5;References;188
42;Immunohistological study on stro-1 in developing rat dental tissues with light and electron microscopy;190
42.1;1 Introduction;190
42.2;2 Materials and Methods;190
42.3;3 Results;191
42.4;4 Conclusion;191
42.5;References;191
43;The physiological calcification process is replicated in a rat embryonic calvarial culture;192
43.1;1 Introduction;192
43.2;2 Materials and Methods;193
43.3;3 Results;193
43.4;4 Conclusion;193
43.5;References;193
44;Tonometric measurement of the gingiva in young and elder humans;194
44.1;1 Introduction;194
44.2;2 Materials and Methods;195
44.3;Results;195
44.4;4 Discussion and Conclusion;195
44.5;References;196
45;Isolation and comparison of mesenchymal stem cells derived from human wisdom tooth germs and periodontal ligament in vitro;197
45.1;1 Introduction;197
45.2;2 Materials and Methods;198
45.3;3 Results;198
45.4;4 Discussion;198
45.5;References;199
46;Unitary discharges of TMJ mechanosensitive neurons during cortically induced jaw movement;200
46.1;1 Introduction;200
46.2;2 Methods;201
46.3;3 Response Properties of TMJ Units;201
46.4;4 Neuronal Activities of TMJ Neurons During Cortically Induced Jaw Movement;201
46.5;References;202
47;Evaluation of bone metabolism of temporomandibular joint by using high resolution PET scanner;203
47.1;1 Introduction;203
47.2;2 Quantitative Evaluation of Bone Metabolic Activity by Using PET Scanner;204
47.3;3 Bone Metabolic Activity in TMJ Induced by Tooth Support Loss;204
47.4;Reference;205
48;Physiological role of type ii bone morphogenetic protein receptor and its interacting molecules in bone morphogenetic protein ;206
48.1;1 Introduction;207
48.2;2 Regulation of Smad Signaling;207
48.3;3 Regulation of Non-Smad Signaling;207
48.4;References;207
49;Role of the protein serine/threonine phosphatase dullard in cell differentiation;209
49.1;1 Introduction;209
49.2;2 Function of Dullard;210
49.3;3 Regulation of Dullard;210
49.4;References;210
50;The role of extracellular signal-regulated kinase 5 signaling pathway in neurons;212
50.1;1 Introduction;212
50.2;2 ERK5 Pathway;213
50.3;3 Neuronal Survival;213
50.4;References;213
51;Regulation of bone morphogenetic protein-mediated signaling by tumor necrosis factor-.a;215
51.1;1 Introduction;215
51.2;2 Regulation of Genes Encoding BMP2 and BMPRs;216
51.3;3 Regulation of the Gene Encoding Inhibitory Smad;216
51.4;References;216
52;Mechanosensitive TRP channels in osteoblasts;218
52.1;1 Introduction;218
52.2;2 Mechanosensitive TRP Channels;219
52.3;3 Expression Pattern of TRPs in the Osteoblastic Cells;219
52.4;References;219
53;Immunohistochemical localization of CD134 ligand, CD137 ligand, GITR ligand, and BAFF in Sjögren’s syndrome-like autoimmune sia;220
53.1;1 Introduction;220
53.2;2 Materials and Methods;221
53.3;3 Results and Discussion;221
54;Expression of microrna during tooth development;223
54.1;1 Introduction;223
54.2;2 Materials and Methods;224
54.3;3 Results and Discussion;224
54.4;References;224
55;New quantitative fluorometry for evaluating oral bacterial adhesion to biomaterials;226
55.1;1 Introduction;226
55.2;2 The Non-RI Quantitative Method using Alamar Blue.®;227
55.3;Conclusion;227
55.4;References;227
56;Analgesic effects of NOD1 and NOD2 agonists;228
56.1;References;230
57;Porphyromonas gingivalis.-induced alveolar bone loss in interleukin-18 transgenic mice;231
57.1;1 Introduction;232
57.2;2 Materials and Methods;232
57.3;3 Results and Discussion;232
57.4;References;233
58;Anaphylaxis-like shock induced by LPS plus antineutrophil monoclonal antibodies in mice;234
59;Concentrations of metal ions in murine nickel allergy and its cross-reactions: effects of lipopolysaccharide;236
59.1;Reference;237
60;Muramyldipeptide augments the actions of LPS via multiple fashions in mice;238
60.1;1 Introduction;239
60.2;2 Materials and Methods;239
60.3;3 Results and Discussion;239
60.4;References;240
61;Isolation and identification of viable bacteria within acrylic resin denture bases;241
61.1;1 Introduction;241
61.2;2 Detection of Viable Bacteria within Acrylic Resin Denture Bases;242
61.3;3 Identification of Bacteria Isolated within Denture Bases;242
61.4;Clinical Implication of Acrylic Resin Denture;242
61.5;References;242
62;Bactericidal effect of photodynamic therapy;243
62.1;References;244
63;Induction of Tregs from PBMC by interacting with immunosuppressive molecule B7-H3 on oral mesenchymal stem cells;245
63.1;Introduction;245
63.2;2 Materials and Methods;246
63.3;3 Results and Discussion;246
63.4;4 Conclusion;246
63.5;References;247
64;A method for determining the profiles of biomass volume and glucan within dental plaque;248
64.1;1 Introduction;248
64.2;2 Materials and Methods;248
64.3;3 Results and Discussion;249
64.4;References;250
65;Porphyromonas gingivalis. is widely distributed in subgingival plaque biofilm of elderly subjects;251
65.1;1 Introduction;251
65.2;2 Relationship Between Periodontal Status and .P. gingivalis. in Subgingival Plaque Biofilm of Elderly Subjects;252
65.3;3 Inhabitation of .P. gingivalis. in Subgingival Plaque of Elderly Subjects;252
65.4;4 Clinical Implication;252
65.5;References;253
66;Profiling of dental plaque microflora on root caries lesions and the protein-degrading activity of these bacteria;254
66.1;1 Introduction;254
66.2;2 Predominant Bacteria in Plaque on Root Caries;255
66.3;3 Protein-Degrading Bacteria in Plaque on Root Caries;255
66.4;4 Conclusions;255
66.5;References;255
67;Characterization of glucosyltransferases synthesizing (1.6)-.a.-.d.-glucan from .Streptococcus sobrinus. and .Streptococcus do;256
67.1;1 Introduction;256
67.2;2 Experiments;257
67.2.1;2.1 Purification and Properties of GTF-T;257
67.3;3 Molecular Properties of GTF-T;257
67.4;4 Linkage Analysis of Glucans Synthesized by GTF-T;257
67.5;5 Conclusion;257
67.6;References;258
68;Profiling of dental plaque biofilm on first molars with orthodontic bands and brackets;259
68.1;1 Introduction;259
68.2;2 Profiling of Plaque Biofilm on Orthodontic Appliances;260
68.3;3 Clinical Implication;260
68.4;References;260
69;Hydrogen-sulfide production from various substrates by oral .Veillonella. and effects of lactate on the production;261
69.1;1 Introduction;261
69.2;2 H.2.S Production from Various Sulfur Compounds and Effects of Lactate on H.2.S Production;262
69.3;References;262
70;Denture plaque removal efficacy of denture cleansing device utilizing radical disinfection ability of activated low concentrati;263
70.1;1 Introduction;263
70.2;2 Materials and Methods;264
70.3;3 Results;264
70.4;4 Discussion;264
70.5;References;265
71;Detection of herpes simplex virus type 1 in human cadaver trigeminal ganglia;266
71.1;1 Introduction;266
71.2;2 Methods;267
71.3;3 Results and Discussion;267
71.4;Reference;267
72;Transmitted laser beam power of the resin washed by experimental washing machine for dentures;268
73;The evaluation of the dental disinfection device with low concentration of H.2.O.2. and laser diode;270
73.1;1 Introduction;270
73.2;2 Materials and Methods;271
73.3;3 Results;271
73.4;4 Discussion;271
73.5;References;272
74;Rapid identification of HACEK group bacteria using 16S rRNA gene PCR-RFLP;273
74.1;Reference;275
75;A novel aspartate-specific dipeptidylpeptidase produced from .Porphyromonas endodontalis;276
75.1;Reference;277
76;Short-term effect of single NaF-mouthrinse on glucose-induced pH fall in dental plaque;278
76.1;1 Introduction;278
76.2;2 NaF-Mouthrinse Inhibits Plaque Acid Production;279
76.3;3 Fluoride Retained within Dental Plaque Can Inhibit Plaque Acid Production in Acidic Environment;279
76.4;References;279
77;Short-time effect of fluoride on acid production by .Streptococcus mutans;280
77.1;1 Introduction;280
77.2;2 Short-Time Fluoride Exposure Inhibits pH Fall by .Streptococcus mutans;281
77.3;3 Conclusion;281
77.4;References;281
78;Real-time PCR analysis of cariogenic bacteria in supragingival plaque biofilm microflora on caries lesions of children;282
78.1;1 Introduction;283
78.2;2 Relationship Between the Status of Dental Caries and .S. mutans. in Supragingival Plaque;283
78.3;References;283
79;Involvement of cough reflex impairment and silent aspiration of oral bacteria in postoperative pneumonia: a model of aspirat;284
79.1;1 Introduction;285
79.2;2 Relationship Between Involvement of Cough Reflex Impairment and Silent Aspiration of Oral Bacteria;285
79.3;Reference;285
80;The production of secretory leukocyte protease inhibitor from gingival epithelial cells in response to .Porphyromonas gingival;286
80.1;Reference;287
81;Analysis of antigen incorporating and processing cells in sublingal immunotherapy;288
82;Acoustic mineral density measurement to evaluate clinical demineralized lesions;290
82.1;1 Objective;290
82.2;2 Methods;291
82.3;3 Results and Discussions;291
83;Experimental Ti–Ag alloys inhibit biofilm formation;293
83.1;1 Introduction;293
83.2;2 Biofilm Inhibition: An Additional Characteristic of Ti–Ag Alloys;294
83.3;3 Conclusion;294
83.4;References;295
84;Apatite formation from octacalcium phosphate with fluoride;296
84.1;1 Introduction;296
84.2;2 Hydrolysis of OCP with Fluoride;297
84.3;3 The character of the Hydrolyzed Product;297
84.4;References;297
85;Effect of topography of the octacalcium phosphate granule surfaces on its bone regenerative property;298
85.1;1 Introduction;299
85.2;2 Characterization of OCP Granules and Implant Procedure;299
85.3;3 Effect of Surface Topography for the Osteogenic Capability of OCP Granules;299
85.4;References;300
86;The influence of sericin solution on wettability and antifungal effect of resin surface;301
86.1;1 Background;301
86.2;2 Material and Methods;302
86.3;3 Results and Discussion;302
86.4;4 Conclusions;303
87;Adhesives and resin composites as functional units;304
87.1;1 Introduction;304
87.2;2 Shear Bond Strength to Dentin;305
87.3;3 Marginal Gap Width and Polymerization Contraction Stress;305
87.4;4 Conclusions;306
87.5;References;306
88;Effects of bisphosphonates on bone marrow stromal cells;307
88.1;1 Introduction;307
88.2;2 Bisphosphonate Zoledronate;308
88.3;3 Bisphosphonate Clodronate;308
88.4;4 Bisphosphonates (Alendronate, Risedronate, or Zoledronate);308
88.5;References;309
89;Tooth shape reconstruction from dental micro CT images;310
89.1;1 Introduction;310
89.2;2 Materials and Methods;311
89.3;3 Results;311
89.4;4 Discussion and Conclusion;311
90;Formation of hydroxyapatite film on tooth using powder-jet-deposition;312
90.1;1 Introduction;312
90.2;Materials and Methods;313
90.3;Results;313
90.4;Discussions;313
90.5;Conclusions;314
90.6;Reference;314
91;Electrodeposition of apatite onto titanium substrates under pulse current;315
91.1;1 Introduction;315
91.2;2 Electrodeposition of Apatite onto Ti Substrates;316
91.3;3 Structure of Apatite Electrodeposited on Ti Substrates;316
91.4;4 Adhesion Between Apatite and Ti Substrates;316
92;Alginate/octacalcium phosphate composites enhance bone formation in critical-sized mouse calvaria defects;318
92.1;1 Introduction;318
92.2;2 Materials and Methods;319
92.2.1;2.1 Preparation and Characterization of Alg/OCP Composites;319
92.2.2;2.2 Cell Culture and Assay of Cell Proliferation in the Alg/OCP Disks;319
92.2.3;2.3 Implantation Procedure;319
92.3;3 Results and Discussions;319
92.4;References;320
93;Strength of porcelain fused to Ti-20%Ag alloy made by CAD/CAM;321
93.1;1 Introduction;321
93.2;References;322
94;Effect of various solutions to exudation of internal fluids from dentinal tubules;323
94.1;1 Introduction;323
94.2;2 Materials and Methods;324
94.3;3 Results;324
94.4;4 Discussion and Conclusion;324
94.5;Reference;324
95;Evaluation of retentive force of .b.-type Ti–6Mo–4Sn alloy wire to apply for the abutment tooth of removable partial denture;325
95.1;1 Introduction;325
95.2;2 Materials and Methods;326
95.3;3 Results;326
95.4;4 Discussion;326
95.5;5 Conclusions;326
95.6;References;327
96;Medical application of magnesium and its alloys as degradable biomaterials;328
96.1;1 Introduction;329
96.2;2 Biodegradation of Magnesium Alloys;329
96.3;3 Biocompatibility of Magnesium;329
96.4;4 Medical Application of Magnesium Alloys;329
96.5;References;330
97;Difference between age generation of oral health examination in a rural town;332
97.1;1 Introduction;333
97.2;2 Aim;333
97.3;3 Methods;333
97.4;4 Result;333
97.5;5 Conclusion;333
97.6;Reference;334
98;Impact of oral health status on healthy life expectancy in community-dwelling population: The AGES Project cohort study;335
98.1;1 Introduction;336
98.2;2 Methods;336
98.3;3 Results;336
98.4;4 Conclusion;336
98.5;References;337
99;Wireless magnetic motion capture system for medical use;338
99.1;1 Introduction;339
99.2;2 System Setup and Position Detection;339
99.3;3 Conclusion;339
99.4;References;339
100;Evaluation of the optimal time of the dental treatment for the elderly;341
100.1;1 Introduction;342
100.2;2 Materials and Methods;342
100.3;3 Results and Discussion;342
100.4;4 Conclusions;343
100.5;References;343
101;Educational effect on tooth preparation of visual feedback using computer graphics;344
101.1;1 Introduction;344
101.2;2 Research Projects;344
101.3;3 Conclusion;345
101.4;References;345
102;Japanese men OSAHS patient’s anatomical features;346
102.1;1 Introduction;346
102.2;2 Material and method;347
102.3;3 Results and discussion;348
102.4;Reference;349
103;Association between periodontal disease and risk for atherosclerosis in hypertensive patients;350
103.1;1 Introduction;350
103.2;2 Materials and Methods;351
103.3;3 Results;351
103.4;4 Discussion;351
103.5;References;352
104;Leading a patient to a dental office: the evaluation of pain and stress during the dental treatment using an air-pad sensor sy;353
104.1;1 Purpose;353
104.2;2 Materials and methods;354
104.3;3 Results;354
104.4;Conclusion;354
104.5;Reference;354
105;Can symptom awareness of the elderly be a clue to find oral diseases and promote oral health behaviors?;355
105.1;1 Introduction;356
105.2;2 Symptom awareness;356
105.3;3 Relationship between symptoms and oral diseases;356
105.4;4 Relationship between those symptoms and oral health behaviors;356
105.5;5 Conclusions;356
105.6;References;357
106;The study of mandibular position applied to oral appliance for treatment of obstructive sleep apnea syndrome;358
106.1;1 Introduction;358
106.2;2 Recordings of GG activity at predetermined mandibular position;358
106.3;3 Relationship between GG activity and mandibular position;359
107;Prediction of future number of remaining teeth of Japanese elderly, based on data from the national survey of dental diseases i;360
107.1;1 Introduction;360
107.2;2 Materials and methods;361
107.3;3 Results and discussion;361
108;National survey on the school-based fluoride mouth rinsing program in Japan: proposition regarding final assessment of Healthy ;362
108.1;1 Introduction;363
108.2;2 Methods;363
108.3;3 Results and discussion;363
109;A new intra-oral pressure monitor for screening swallowing dysfunction;365
109.1;1 Introduction;365
109.2;2 Relation between positive and negative intra-oral pressure;366
109.3;3 Monitoring the developing process of swallowing;366
109.4;4 Need for screening device in general health check-up;366
109.5;5 Conclusion;366
110;A numerical simulation method for dental occlusion with forces applied to the tooth in mandible;367
110.1;1 Introduction;367
110.2;2 Numerical method and results;368
110.3;Reference;368
111;Osteopontin and CSF-1 in bone resorption;370
111.1;1 Introduction;370
111.2;2 Osteopontin and bone resorption;371
111.3;3 Osteopontin receptors;371
111.4;4 CSF-1 in tumor cells;372
111.5;5 Conclusion;373
111.6;References;373
112;Role of amelogenin self-assembly in protein-mediated dental enamel formation;376
112.1;1 Introduction;377
112.2;2 Primary sequence and properties of amelogenin;377
112.3;3 Self-assembly of amelogenin;377
112.4;4 Regulation of calcium phosphate crystal growth and organization by amelogenins;379
112.5;5 Conclusions;380
112.6;References;380
113;The human genetics of amelogenesis imperfecta;382
113.1;1 Introduction;382
113.2;2 The genetics of amelogenesis imperfecta;383
113.2.1;2.1 AMELX. (MIM 300391);383
113.2.2;2.2 ENAM. (MIM 606585);384
113.2.3;2.3 AMBN. (MIM 601259);384
113.2.4;2.4 MMP20. (MIM 604629);384
113.2.5;2.5 KLK4. (MIM 603767);385
113.2.6;2.6 FAM83H. (MIM 611927);385
113.2.7;2.7 DLX3. (MIM 600525);386
113.2.8;2.8 CNNM4. (MIM 607805);386
113.3;3 Summary;386
113.4;References;387
114;Porphyromonas gingivalis.: surface polysaccharides as virulence determinants;389
114.1;1 Introduction;389
114.2;2 Exopolysaccharide and lipopolysaccharides;390
114.3;3 K-antigen capsule;391
114.4;4 Regulation of surface polysaccharide expression;392
114.5;References;393
115;Building the genomic base-layer of the oral “omic” world;395
115.1;1 Introduction;395
115.2;References;399
116;Cariogenic microflora and the immune response;401
116.1;1 Introduction;401
116.2;2 Natural History of Mutans Streptococcal Infection;402
116.3;3 Ontogeny of Mucosal Immunity on the Oral Cavity;402
116.4;4 Active and Passive Vaccines Against Dental Caries;404
116.5;References;405
117;Porphyromonas gingivalis. infection elicits immune-mediated RANKL-dependent periodontal bone loss in rats;407
117.1;1 Introduction;407
117.2;2 Results;408
117.3;3 Conclusion;408
117.4;Reference;408
118;Is RANKL shedding involved in immune cell-mediated osteoclastogenesis?;410
118.1;1 Introduction;410
118.2;2 RANKL shedding;411
118.3;3 Conclusion;411
118.4;References;412
119;Possible IgG transportation mechanism mediated by neonatal Fc receptor expressed in gingival epithelial cells;413
119.1;1 Introduction;413
119.2;2 Approaches;414
119.3;3 Results;414
119.4;4 Conclusion;415
119.5;References;415
120;Effects of extracellular adenosine on sRANKL production from activated T cells;416
120.1;1 Introduction;416
120.2;2 Methods;417
120.2.1;2.1 Reagents;417
120.2.2;2.2 T lymphocyte stimulation assay;417
120.3;3 Results;418
120.4;4 Conclusion;418
120.5;References;419
121;Activation of the critical enamel protease kallikrein-4;420
121.1;1 Introduction;420
121.2;2 Activation of kallikreins;420
121.3;3 Activation of KLK4 by matrix metalloproteinase-20;421
121.4;4 Activation of KLK4 by dipeptidyl peptidase I;421
121.5;5 Conclusions;421
121.6;References;422
122;Epitopes shared among pioneer oral flora and .Streptococcus mutans. GbpB;423
122.1;1 Narrative;423
122.2;References;424
123;Inhibitory effect of porcine amelogenins on spontaneous mineralization;425
123.1;1 Introduction;425
123.2;2 Methods;426
123.3;3 Results and discussion;426
123.4;References;427
124;A stress-based mechanism to explain dental fluorosis;428
124.1;1 Introduction;428
124.2;2 ER stress and the unfolded protein response;429
124.3;3 Fluoride activates the UPR;429
124.4;4 Conclusions;429
124.5;References;429