Satir / Christensen Cilia

Methods and Protocols
1. Auflage 2016
ISBN: 978-1-4939-3789-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1454, 259 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium , and methods to study centrosomes and cilia in C. elegans and Drosophila . Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Satir, Peter

Christensen, Søren Tvorup

Inhaltsverzeichnis

Methods for Studying Ciliary Import Mechanisms.-  Targeting of ASH Domain-Containing Proteins to the Centrosome.- Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesis and Autophagy.- Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii.-  Methods for Studying Movement of Molecules within Cilia.- A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.-  Kymographic  Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.-  Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium.-  STED and STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.-  Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas.- Planaria as a Model System for the Analysis of Ciliary Assembly and Motility.