Setlow Genetic Engineering: Principles and Methods 28

2007
ISBN: 978-0-387-34504-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 28, 190 Seiten

Reihe: Genetic Engineering: Principles and Methods

This book, published by Springer since 1979, presents state-of-the-art discussions in modern genetics and genetic engineering. This focus affirms a commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among other topics.

Autoren/Hrsg.

Setlow, Jane K.

Inhaltsverzeichnis

1;ACKNOWLEDGMENT;6
2;CONTENTS OF EARLIER VOLUMES;8
3;CONTENTS;21
4;REGULATION OF PLANT INTERCELLULAR COMMUNICATION VIA PLASMODESMATA;23
4.1;INTRODUCTION;23
4.2;PLASMODESMATA: STRUCTURES AND FUNCTIONS;23
4.3;MORE APPROACHES TO IDENTIFY PD COMPONENTS;34
4.4;PERSPECTIVES;34
4.5;ACKNOWLEDGMENTS;35
4.6;REFERENCES;35
5;ROOT-KNOT AND CYST NEMATODE PARASITISM GENES: THE MOLECULAR BASIS OF PLANT PARASITISM;38
5.1;INTRODUCTION;38
5.2;MAJOR SEDENTARY PHYTONEMATODES;39
5.3;ADAPTATIONS FOR PLANT PARASITISM;40
5.4;NEMATODE PARASITISM GENES AND THEIR PRODUCTS;43
5.5;POTENTIAL ROLES OF PARASITISM PROTEINS;45
5.6;PARASITISM GENE IDENTIFICATION;45
5.7;KNOWN PARASITISM GENES;46
5.8;CURRENT HYPOTHESES OF PARASITISM PROTEIN FUNCTIONS;46
5.9;PARASITISM GENES IN A WIDER SENSE;51
5.10;DIFFERENCES BETWEEN ROOT-KNOT NEMATODES AND CYST NEMATODES;52
5.11;WHICH GLAND HAS WHICH FUNCTION?;53
5.12;FUNCTIONAL CHARACTERIZATION OF PARASITISM PROTEINS;54
5.13;PRESENT AND FUTURE;59
5.14;REFERENCES;61
6;MUTAGENESIS OF HUMAN p53 TUMOR SUPPRESSOR GENE SEQUENCES IN EMBRYONIC FIBROBLASTS OF GENETICALLY- ENGINEERED MICE;65
6.1;INTRODUCTION;65
6.2;IMMORTALIZATION MECHANISMS OF MURINE EMBRYONIC FIBROBLASTS( MEFs): CRUCIAL ROLE OF p53 POINT MUTATIONS;67
6.3;HUF (HUPKI EMBRYONIC FIBROBLAST) IMMORTALIZATION AS A METHOD TO SELECT FOR DYSFUNCTIONAL POINT MUTATIONS IN HUMAN P53 GENE SEQUENCES;68
6.4;P53 MUTAGENESIS ASSAY: SHORT PROTOCOL;70
6.5;INDICATORS OF LOSS OF p53 FUNCTION IN HUF CELL LINES;71
6.6;PERSPECTIVES;72
6.7;REFERENCES;73
7;SALICYLIC ACID-, JASMONIC ACID- AND ETHYLENE-MEDIATED REGULATION OF PLANT DEFENSE SIGNALING;75
7.1;INTRODUCTION;75
7.2;EARLY EVENTS FOLLOWING PATHOGEN PERCEPTION;77
7.3;THE SALICYLIC ACID PATHWAY;82
7.4;THE JASMONIC ACID PATHWAY;87
7.5;CONCLUSION;94
7.6;ACKNOWLEDGMENTS;95
7.7;REFERENCES;95
8;PROXIMITY LIGATION: A SPECIFIC AND VERSATILE TOOL FOR THE PROTEOMIC ERA;104
8.1;ABSTRACT;104
8.2;INTRODUCTION;104
8.3;PROXIMITY LIGATION;106
8.4;PROXIMITY-LIGATION IN SITU ASSAY ( P- LISA);108
8.5;CURRENT METHODS TO DETECT PROTEIN INTERACTIONS;108
8.6;DETECTION OF PROTEIN INTERACTIONS AND MODIFICATIONS BY PROXIMITY LIGATION;109
8.7;SUMMARY;110
8.8;ACKNOWLEDGMENTS;111
8.9;REFERENCES;111
9;PROTEIN OVEREXPRESSION IN MAMMALIAN CELL LINES;113
9.1;INTRODUCTION;113
9.2;TRANSIENT VS STABLE EXPRESSION;114
9.3;EXPRESSION FROM CLONED GENOMIC FRAGMENTS;117
9.4;EXPRESSION OF THE HUMAN CXADR GENE IN BAC- TRANSFECTED MOUSE CELLS;118
9.5;CONCLUSIONS;120
9.6;ACKNOWLEDGMENTS;120
9.7;REFERENCES;120
10;A HIGH-THROUGHPUT APPROACH TO PROTEIN STRUCTURE ANALYSIS;123
10.1;INTRODUCTION;124
10.2;TARGET SELECTION;128
10.3;PROTEIN PRODUCTION AND BIOPHYSICAL ANALYSIS;129
10.4;PROTEIN STRUCTURE ANALYSIS;130
10.5;HOMOLOGY MODELING OF REPRESENTATIVE PROTEIN FAMILY MEMBERS;137
10.6;STRUCTURE TO FUNCTION;138
10.7;CONCLUSION;142
10.8;REFERENCES;142
11;NEW MASS-SPECTROMETRY-BASED STRATEGIES FOR LIPIDS;147
11.1;ABSTRACT;147
11.2;INTRODUCTION;148
11.3;MS SAMPLE INTRODUCTION, IONIZATION STRATEGIES, AND SCAN MODES;149
11.4;ESI-MS AND ESI-MS/MS APPROACHES TO LIPIDOMIC ANALYSES;155
11.5;APCI-MS FOR LIPIDOMICS;157
11.6;MALDI-MS FOR LIPIDOMICS;158
11.7;MALDI AND TOF-SECONDARY ION MASS SPECTROMETRY ( TOF- SIMS) FOR LIPID IMAGING;159
11.8;SPECIALIZED DERIVATIZATION TECHNIQUES THAT CAN AID LIPIDOMIC ANALYSES;164
11.9;INFORMATIC CHALLENGES;165
11.10;OUTLOOK;167
11.11;ACKNOWLEDGMENTS;168
11.12;REFERENCES;168
12;PAIRED-END GENOMIC SIGNATURE TAGS: A METHOD FOR THE FUNCTIONAL ANALYSIS OF GENOMES AND EPIGENOMES;176
12.1;INTRODUCTION;176
12.2;WHAT ARE GENOMIC SIGNATURE TAGS?;177
12.3;SERIAL ANALYSIS OF CHROMATIN OCCUPANCY (SACO);178
12.4;PAIRED-END GENOMIC SIGNATURE TAGS (PE-GST);180
12.5;INCREASING TAG LENGTH AT THE 3' END;183
12.6;BSER I DIGESTION TO RELEASE PAIRED- END DITAGS;184
12.7;PAIRED-END PROFILING OF THE METHYLOME;184
12.8;SUMMARY;187
12.9;ACKNOWLEDGMENTS;188
12.10;REFERENCES;188
13;INDEX;191