Söling / Willms Regulation of Gluconeogenesis

9th Conference of the Gesellschaft für Biologische Chemie
1. Auflage 2013
ISBN: 978-1-4832-6200-0
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 360 Seiten, Web PDF

Regulation of Gluconeogenesis documents the proceedings of 9th Conference of the Gesellschaft für Biologische Chemie. This meeting was first planned as a continuation of a former Conference of the Gesellschaft für Biologische Chemie on ''Stoffwechsel der isoliert perfundierten Leber''. However, the organizers of the present meeting decided that it seemed far more reasonable to restrict the subject to a special biochemical problem than to a technical procedure which had not changed very much during the last years. This volume contains 24 chapters and begins with a paper that identifies the substrates, activators, and inhibitors which may influence the rate of reaction of pyruvate carboxylase from liver. Other papers deal with topics such as purification and properties of pyruvate carboxylase from rat liver; the location of pyruvate carboxylase in mitochondria; effects of quinolinic acid on the free and total nicotinamide-adenine dinucleotides of rat liver; and effects of biguanides on gluconeogenesis in rats and guinea pigs.

Autoren/Hrsg.

Söling, Hans-Dieter

Willms, Berend

Inhaltsverzeichnis

1;Front Cover;1
2;Regulation of Gluconeogenesis;4
3;Copyright Page;5
4;Table of Contents;12
5;Preface;6
6;Contributing Authors and Discussants;9
7;Chapter 1. Possible Control Mechanisms of Liver Pyruvate Carboxylase;14
7.1;Summary;14
7.2;Introduction;15
7.3;Activation by Acetyl-CoA;18
7.4;Inhibitors of Pyruvate Carboxylase;20
7.5;Acknowledgment;23
7.6;References;23
8;Chapter 2. Purification and Properties of Pyruvate Carboxylase from Rat Liver;24
8.1;Summary;24
8.2;Introduction;24
8.3;Results;25
8.4;References;32
9;Chapter 3. Comparative Studies with Pyruvate Carboxylase from Rat and Guinea Pig Liver;33
9.1;References;37
9.2;Discussion to Utter and Fung, and to Seufert, Herlemann and Seubert;38
10;Chapter 4. On the Intracellular Location of Pyruvate Carboxylase;42
10.1;Summary;42
10.2;Introduction;42
10.3;Results and Discussion;43
10.4;References;53
10.5;Announced Discussion;54
10.6;Reference;55
10.7;Announced Discussion;57
10.8;References;58
10.9;Discussion to Weiss, Only, Dugal and Seubert;60
11;Chapter 5. Regulation of Pyruvate Metabolism in Rat Liver Mitochondria;63
11.1;Summary;63
11.2;Introduction;63
11.3;Materials and Methods;64
11.4;Results and Discussion;64
11.5;References;72
11.6;Discussion to Walter and Stucki;73
12;Chapter 6. Activities of Pyruvate Carboxylase, Phosphoenolpyruvate Carboxykinase and Pyruvate Kinase of New Zealand Obese+(NZO) Mice Livers During Different Phases of Diabetes after Starvation, Cortisol and Insulin Treatment.;76
12.1;Summary;76
12.2;Introduction;76
12.3;Materials and Methods;76
12.4;Enzyme Assays;77
12.5;Results and Discussion;77
12.6;References;78
13;Chapter 7. The Development of Gluconeogenic Function in Rat Liver;79
13.1;Summary;79
13.2;Introduction;80
13.3;Glucagon Experiments;85
13.4;Ether Anesthesia and Gluconeogenesis;86
13.5;Conclusions;93
13.6;Acknowledgment;93
13.7;References;93
13.8;Discussion to Stornier and Staib, and to Ballard and Philippidis;94
13.9;General Discussion;97
14;Chapter 8. Effects of Quinolinic Acid on the Free and Total Nicotinamide-Adenine Dinucleotides of Rat Liver;105
14.1;Summary;105
14.2;Introduction;106
14.3;Materials and Methods;106
14.4;Results;106
14.5;Discussion;113
14.6;Acknowledgments;113
14.7;References;114
14.8;Discussion to Williamson, Mayor and Veloso;114
15;Chapter 9. On the Redox State of NADYNADH Systems in Guinea Pig Liver Under Different Experimental Conditions;116
15.1;Summary;116
15.2;+ Unusual Abbreviations:.;116
15.3;Results;117
15.4;Acknowledgments;126
15.5;References;126
16;Chapter 10. Gluconeogenesis and Redox State;127
16.1;Summary;127
16.2;References;130
16.3;Discussion to Willms, Kleineke and Söling, and Krebs;131
17;Chapter 11. On the Inhibition of Gluconeogenesis by 1-ß-Phenyl-Ethylbiguanide in the Perfused Guinea Pig Liver;140
17.1;Summary;140
17.2;Introduction;140
17.3;Results and Discussion;141
17.4;References;152
18;Chapter 12. Effects of Biguanides on Gluconeogenesis in Rats and Guinea Pigs;153
18.1;Discussion to Haeckel and Haeckel;154
19;Chapter 13. Regulation of Gluconeogenesis with Ethanol and Fructose by the Isolated Perfused Rat Liver;158
19.1;Summary;158
19.2;Introduction;158
19.3;Methods;159
19.4;Results and Discussion;160
19.5;Experiments with Ethanol;160
19.6;Experiments with Fructose;161
19.7;Experiments with Ethanol and Fructose;164
19.8;Acknowledgment;165
19.9;References;165
20;Chapter 14. Fructose as a Precursor for Ketogenesis;166
20.1;References;169
20.2;Discussion to Papenberg;170
21;Chapter 15. Mechanism of Glucagon Activation of Gluconeogenesis;173
21.1;Summary;173
21.2;Introduction;174
21.3;Materials and Methods;174
21.4;Results;176
21.5;Discussion;184
21.6;Acknowledgment;190
21.7;References;190
22;Chapter 16. Dissociation of Gluconeogenic and Ketogenic Action of Glucagon in the Perfused Rat Liver;192
22.1;Summary;192
22.2;Introduction;193
22.3;Methods;193
22.4;Results and Discussion;194
22.5;A cknowledgment;202
22.6;References;202
22.7;Discussion to Exton, Ui and Park and Fröhlich and Wieland;204
22.8;Summary;207
22.9;Introduction;208
22.10;Materials and Methods;208
22.11;Reagents;209
22.12;Results and Discussion;209
22.13;Acknowledgment;219
22.14;References;219
22.15;Discussion to Ruderman, Toews, Lowy and Shafrir;220
23;Chapter 17. Regulation of Gluconeogenesis in Rat and Guinea Pig Liver;223
23.1;Summary;223
23.2;Introduction;224
23.3;Conclusions;234
23.4;Acknowledgment;235
23.5;References;237
23.6;Discussion to Söling, Willms and Kleineke;238
24;Chapter 18. The Effect of 3', 5'-cyclic AMP on Glucose Synthesis in Isolated Rat Kidney Tubules;239
24.1;Summary;239
24.2;Introduction;239
24.3;Methods;240
24.4;Results;241
24.5;Discussion;247
24.6;Acknowledgment;248
24.7;References;248
24.8;Discussion to Guder and Wieland;249
25;Chapter 19. Effect of Proinsulin on the Metabolism of Alanine in Isolated Perfused Rat Livers;250
25.1;Summary;250
25.2;Introduction;250
25.3;Methods;251
25.4;Results;252
25.5;Discussion;252
25.6;References;257
25.7;Discussion to Rudorff, Windeck and Staib;258
25.8;General Discussion;259
26;Chapter 20. The Role of Pyruvate Carboxylase and P-enolpyruvate Carboxykinase in Rat Adipose Tissue;268
26.1;Summary;268
26.2;Pyruvate Carboxylase;269
26.3;Tissue Distribution and Properties of the Adipose Tissue Enzyme;269
26.4;P-enolpyruvate Carboxykinase;278
26.5;Conclusions;285
26.6;Acknowledgment;286
26.7;References;286
26.8;Discussion to Hanson, Patel, Reshef and Ballard;287
27;Chapter 21. Stimulation of Amino Acid Metabolism and Gluconeogenesis from Amino Acids in the Liver of Fasting Rats Treated with Antilipolytic Agents;289
27.1;Summary;289
27.2;Introduction;290
27.3;Results;291
27.4;Discussion;297
27.5;References;298
27.6;Discussion to Hasselblatt, Panten and Poser;298
27.7;References;301
28;Chapter 22. Transfer of Carbon and Hydrogen Across the Mitochondrial Membrane in the Control of Gluconeogenesis;306
28.1;Summary;306
28.2;Introduction;307
28.3;Acknowledgments;325
28.4;References;325
28.5;Discussion to Williamson;326
29;Chapter 23. Interaction of Gluconeogenesis with Mixed Function Oxidation in Perfused Rat Liver;328
29.1;Summary;328
29.2;Introduction;329
29.3;Summary and Conclusions;336
29.4;Acknowledgments;339
29.5;References;339
29.6;Discussion to Thurman and Scholz;340
30;Chapter 24. Some Aspects of Gluconeogenesis from Pyruvate in Hemoglobinfree Perfused Rat Liver;341
30.1;Summary;341
30.2;Perfusion System;342
30.3;Interaction of the Gluconeogenic Pathway with Other Metabolic Pathways of the Cell;344
30.4;14 Mathematical Formulation of the Influence of the TG Y-cycle on the C-Labeling Pattern of Glucose;346
30.5;Pyruvate Permeation through the Liver Cell Membrane;348
30.6;14 C- Activities of Other Intracellular Metabolites 14;348
30.7;Acknowledgments;350
30.8;References;350
30.9;Discussion to Müllhofer and Kuntzen;351
30.10;General Discussion;354