Songyang Telomeres and Telomerase

Methods and Protocols
3rd Auflage 2017
ISBN: 978-1-4939-6892-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1587, 218 Seiten, eBook

Reihe: Methods in Molecular Biology

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition  is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Songyang, Zhou

Inhaltsverzeichnis

Introduction to Telomeres and Telomerase.- Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9.- Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH).- Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH).- Telomere G-Rich Overhang Length Measurement: DSN Method.- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA.- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In.- Analysis of Yeast Telomerase by Primer Extension Assays.- Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP).- Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™).- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques.- Cytogenetic Analysis of Telomere Dysfunction.- Probing the Telomere Damage Response.- Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins.- Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein–Protein Interactions.- In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits.- Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.- Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization.- Analysis of Telomere Proteins by ChromatinImmunoprecipitation (ChIP).