Turksen Stem Cells and Aging

Methods and Protocols
Third Auflage 2025
ISBN: 978-1-0716-4718-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 306 Seiten

Reihe: Springer Protocols

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful   series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date,   serves as an ideal guide for researchers working toward furthering our understanding of the aging process.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Turksen, Kursad

Inhaltsverzeichnis

Protocols to Assess the Aging of Human Mesenchymal Stem Cells.- Analyzing Muscle Stem Cell Function Ex Vivo.- Tubular Aggregates as a Marker of Aging in Skeletal Muscle.- Fusion of Human Synovium-Derived Mesenchymal Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified Adherence Method (MAM).- Multiplex PCR for the Rapid Diagnosis of Myeloproliferative Neoplasms.- Ex Vivo Expansion of Cord Blood Hematopoietic Stem and Progenitor Cells.- Evaluation of Anti-Aging Agents Using the D-Galactose-Induced Accelerated Aging Model.- Methods to Detect and Compare Cellular and Mitochondrial Changes in Senescent and Healthy Mesenchymal Stem Cells.- Transfer of Mitochondria from Healthy Stem Cells to Injured Cells in Stroke with Retinal Impairments.- A Protocol for Detecting DNA Methylation Changes at CpG Sites of Stemness-Related Genes in Aging Stem Cells.- Robust Expansion of Hematopoietic Stem Cells Ex Vivo Using Small Molecule Cocktails.- A Genome-Wide CRISPR/Cas9 Screen Identifies Regulatory Genes for Stem Cell Aging.- Immunocompetent  Brain  Organoids  with  Microglia  Allow  Advanced  Aging  Research.- Quality Control in Human Adipose-Derived Stromal/Stem Cells and Tissue Engineering Fat Models for Aging Studies.- Mesenchymal Stromal Cell (MSC) Isolation and Induction of Acute and Replicative Senescence.- Delivery of Stem Cell Rejuvenating Compounds via Subcutaneous Osmotic Pumps.- Isolation Procedure for Rat Pancreatic Ductal Cells.- Polychromatic Flow Cytometry to Identify Rare Aged Hematopoietic Stem Cell Subpopulations.- Isolation of Human Hematopoietic Stem Cells from an Apheresis Sample.- Quantifying Muscle Regeneration: Activated Muscle Satellite Cells and New Regenerated Myofibers in Chronic and Acute Degeneration Models.- Identification of De Novo Dividing Stem Cells.- Rat Ductal Cell-Derived Differentiation into Islet-Like Cells.