Turksen Stem Cells and Aging

Methods and Protocols
2. Auflage 2019
ISBN: 978-1-4939-9713-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2045, 354 Seiten, eBook

Reihe: Methods in Molecular Biology

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Turksen, Kursad

Inhaltsverzeichnis

Assessing Muscle Stem Cell Clonal Complexity during Aging.- Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.- Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle.- Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue.- Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures.- Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells.- Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis.- Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells.- Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis.- 3D-Embedded Cell Cultures to Study Tendon Biology.- Targeted, Amplicon-Based,Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis.- Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants.- Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreER T2 /Rosa26YFP Mice.- Infrared Spectroscopy and Imaging in Stem Cells and Aging Research.- Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres.- Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry.- Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry.- Methods for Detection of Autophagy in Mammalian Cells.- Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells.- Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer.- Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD).- Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury.- 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol.- Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments.- CRISPR Base Editing in Induced Pluripotent Stem Cells.