Unknown Microbial Gene Techniques, Part B

Molecular Microbiology Techniques
1. Auflage 1995
ISBN: 978-0-08-053639-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 6, 487 Seiten, Web PDF

Reihe: Methods in Molecular Genetics

Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.* * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.

Autoren/Hrsg.

Unknown, Author

Inhaltsverzeichnis

1;Front Cover;1
2;Microbial Gene Techniques;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 6;10
6;Preface;16
7;Volumes in Series;18
8;Section I: Molecular Genetics of Eukaryotic Microbes;20
8.1;Chapter 1. Forward and Reverse Genetics in the Study of the Obligate, Intracellular Parasite Toxoplasma gondii;22
8.2;Chapter 2. Techniques Associated with Protozoan Parasite Genome Analysis;49
8.3;Chapter 3. Aspergillus nidulans as a Model System for Cell and Molecular Biology Studies;67
8.4;Chapter 4. Special Molecular Techniques for Study of Filamentous Fungi;85
9;Section II: Yeast Chromosomes: Transcription, Recombination, Replication;98
9.1;Chapter 5. In Vitro Fragmentation of Yeast Chromosomes and Yeast Artificial Chromosomes at Artificially Inserted Sites and Applications to Genome Mapping;100
9.2;Chapter 6. Basic Analysis of Transcription Factor Binding to Nucleosomes;127
9.3;Chapter 7. Experimental Analysis of Transcription Factor–Nucleosome Interactions;148
9.4;Chapter 8. In Vivo Analysis of Nucleosome Structure and Transcription Factor Binding in Saccharomyces cerevisiae;172
9.5;Chapter 9. Mapping of Yeast Nucleosomes in Vivo;187
9.6;Chapter 10. Novel Assays for Ligation and Cleavage Activities of Site-Specific Recombinases;205
9.7;Chapter 11. Physical Monitoring of Mitotic and Meiotic Recombination in Saccharomyces cerevisiae;223
9.8;Chapter 12. Genetic Dissection of Complex Heteromultimeric Enzymes: The Case of Yeast DNA-Dependent RNA Polymerases;246
9.9;Chapter 13. Pheromone Procedures in Fission Yeast;266
10;Section III: Bacterial Gene Structure and Regulation;284
10.1;Chapter 14. Use of DNA Methylation Deficient Strains in Molecular Genetics;286
10.2;Chapter 15. Identifying the Ends of Infrequent RNA Molecules in Bacteria;299
10.3;Chapter 16. Identification of rcs Genes in Escherichia coli 09:K30:H12 and Involvement in Regulation of Expression of Group IAK30 Capsular Polysaccharide;320
10.4;Chapter 17. Genetic Mapping in Bacillus subtilis;342
10.5;Chapter 18. Molecular and Biochemical Methods for Studying Chemotaxis in Bacillus subtilis;358
10.6;Chapter 19. Cloning of Thermomonospora fusca Cellulase Genes in Escherichia coli and Streptomyces lividans;386
10.7;Chapter 20. Computational Analyses Aiding Identification and Characterization of Proteins, Genes, and Operons;394
11;Section IV: Plasmids and Phages: Replication,Transcription, Assembly;406
11.1;Chapter 21. Techniques to Isolate Specialized Templates for in Vitro Transcription Assays;408
11.2;Chapter 22. Protein–Protein Interactions of DNA-Binding Proteins: Studies on the Replication Initiator Protein, Rep A, of Plasmid P1;419
11.3;Chapter 23. Transcriptional Regulators: Protein–DNA Complexes and Regulatory Mechanisms;440
11.4;Chapter 24. Use of Coliphage a and Other Bacteriophages for Molecular Genetic Analysis of Erwinia and Related Gram-Negative Bacteria;458
11.5;Chapter 25. Isolation, Purification, and Function of Assembly Intermediates and Subviral Particles of Bacteriophages PRD1 and ø6;474
12;Index;494