Unknown Viral Gene Techniques

1. Auflage 1995
ISBN: 978-0-08-053640-8
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 7, 416 Seiten, Web PDF

Reihe: Methods in Molecular Genetics

Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.* Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)

Autoren/Hrsg.

Unknown, Author

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 7;10
4;Preface;16
5;Volumes in Series;18
6;Section I: Viral Vectors;20
6.1;Chapter 1. Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy;22
6.2;Chapter 2. Techniques for Human Adenovirus Vector Construction and Characterization;32
6.3;Chapter 3. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements;50
6.4;Chapter 4. Construction and Use of Recombinant Vaccinia Virus Vectors;64
6.5;Chapter 5. Epstein–Barr Viral Plasmid Vectors and Their Amplifiable Derivatives;84
7;Section II: DNA Viruses;106
7.1;Chapter 6. Methods to Study Epstein–Barr Virus and p53 Status in Human Cells;108
7.2;Chapter 7. The SV40 Minichromosome;120
7.3;Chapter 8. Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Virus Type 1 Genome Using a Cell-Free Cre-lox Recombination System;133
7.4;Chapter 9. Strategies for Studying Transcription Regulation of Neurotropic Viruses in the Central Nervous System: Lesson from JC Virus;150
7.5;Chapter 10. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitis B Viral X and 3'-Truncated S Transactivators;171
7.6;Chapter 11. Investigations on Virus-Host Interactions: An Abortive System;186
8;Section III: Human Immunodeficiency Virus/Retroviruses;212
8.1;Chapter 12. Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA;214
8.2;Chapter 13. Quantitation of HIV-1 RNA in Plasma or Serum Samples;228
8.3;Chapter 14. Detection of Cell Fusion Mediated by the Envelopes of Human Retroviruses by Transactivation of a Reporter Gene;237
8.4;Chapter 15. Assembly of Recombinant Retroviral Gag Precursors into Pseudovirions in the Baculovirus–Insect Cell Expression System;256
8.5;Chapter 16. Retrovirus Reverse Transcription and Integration;273
8.6;Chapter 17. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Virus Type I Tax Protein;286
8.7;Chapter 18. Polymerase Chain Reaction-Based Techniques for Utilizing Retroviruses as Cell Lineage Markers;299
9;Section IV: RNA Viruses;316
9.1;Chapter 19. New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA;318
9.2;Chapter 20. Molecular Studies of Hepatitis Delta Virus RNAs;334
9.3;Chapter 21. Systems to Express Recombinant RNA Molecules by the Influenza A Virus Polymerase in Vivo;348
9.4;Chapter 22. Analysis of Membrane Association in Vivo and Targeting of Viral Proteins in Polarized Epithelial Cells;362
9.5;Chapter 23. Cloning of Viral Double-Stranded RNA Genomes by Single Primer Amplification;378
9.6;Chapter 24. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins;392
9.7;Chapter 25. Protein RNA-Binding Activity Measured by Northwestern Blot Analysis: The Interferon-Inducible RNA-Dependent Protein Kinase PKR;407
10;Erratum;423
11;Index;424