Vainio Kidney Organogenesis

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9021-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 248 Seiten

Reihe: Springer Protocols

This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic 
embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Vainio, Seppo

Inhaltsverzeichnis

Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development.- Mouse Ex Vivo Kidney Culture Methods.- Serum-Free Organ Culture of the Embryonic Mouse Ureter.- Experimental Tubulogenesis Induction Model in the Mouse.- Dissociation of Tissues for Single Cell Analysis.- Long Term Culture of Nephron Progenitor Cells Ex Vivo.- 3D Cell Culture Models of Epithelial Tissues.- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells.- Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.- Protocol to Generate Ureteric Bud Structures from Human iPS Cells.- Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture.- Virus as Renal Functional Genetic Tools.- Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines.- Time-Lapse Technologies and 4D Imaging of Kidney Development.- Decellularizing and Recellularizing Adult Mouse Kidneys.- Optical Projection Tomography Imaging to Study Kidney Organogenesis.- Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model.- Analysis of the Teashirt Target Genes in Ureteric Bud Development.- Methods of Computational Analysis in Kidney Development.