Wang / Wölfl / Jiang New Antibody Microarray Tube for Cellular Localization and Signaling Pathways

1. Auflage 2009
ISBN: 978-3-540-74341-5
Verlag: Springer-Verlag
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 134 Seiten

New Antibody Microarray Tube for Cellular Localization and Signaling Pathways describes a new integrated protein biochip system including protein localization, biocomputing and signaling pathways. In the post-genome era, proteomics study for direct analysis of a group of proteins becomes more and more important. Identification of its location often provides crucial information for understanding the function or regulation of a given protein in biological pathways. The book discusses a new microarray tube with incorporation of nuclear and cytoplasmic protein purification and biocomputing technologies, intended for setting-up signaling pathways and making new discoveries.

The book is intended for researchers in the fields of medicine, biology, electronics, pharmacy, computer science and mathematics. Lin Wang is an associate professor at the Biomedical Engineering Center, Beijing University of Posts and Telecommunications, China.

Autoren/Hrsg.

Wang, Lin

Wölfl, Stefan

Jiang, Minghu

Inhaltsverzeichnis

1;Chapter 1 Introduction;5
1.1;1.1 Antibody Array Technologies and Applications;5
1.2;1.2 Protein Phosphorylation, Localization and Function;7
1.3;1.3 Interaction Proteomics and Pathway Building;8
2;Chapter 2 Technologies;16
2.1;2.1 Materials;16
2.2;2.2 Methods;18
2.3;2.3 Silver Staining in ArrayTube;21
3;Chapter 3 New Cytoplasmic and Nuclear BMP2- Signaling Pattern in U937 Cells;27
3.1;3.1 3.1 Modulation of Phosphorylation and Localization in BMP2- Treated U937 Cells;27
3.2;3.2 BMP2 Signaling in U937 Cells;36
3.3;3.3 BMP2 Signaling Model in U937 Cells;42
4;Chapter 4 New Cytoplasmic and Nuclear BMP2- Signaling in MCF7 Cells;44
4.1;4.1 Modulation of Phosphorylation and Localization in BMP2- Treated MCF7 Cells;44
4.2;4.2 BMP2 Signaling in MCF7 Cells;52
4.3;4.3 BMP2 Signaling Model in MCF7 Cells;54
5;Chapter 5 New Cytoplasmic and Nuclear STI571 Signaling in K562 Cells;56
5.1;5.1 Modulation of Phosphorylation and Localization in STI571- Treated K562 Cells;56
5.2;5.2 STI571 Signaling in K562 Cells;62
5.3;5.3 STI571 Signaling Model in K562 Cells;65
6;Chapter 6 Positive and Negative Co-localized Rela-tionships in Three Treated Cell Lines;67
6.1;6.1 Positive and Negative Co-localized Distributions;67
6.2;6.2 Possible Relationships;87
7;Chapter 7 Cytoplasmic Signaling in Three Treated Cell Lines;91
7.1;7.1 Cytoplasmic Distribution;91
7.2;7.2 Co-cytoplasmic Signaling;96
8;Chapter 8 Proteomic Silencing in Treated Human Cancer Cell Lines;99
8.1;8.1 Proteomic Silence Distributions;99
8.2;8.2 Possible Relationships;102
9;Chapter 9 Signaling Pathway Setting-up Based on Bioinformatics;104
9.1;9.1 Methods;104
9.2;9.2 Results;110
9.3;9.3 Discussion;121
9.4;9.4 Conclusions;122
10;References;123