E-Book, Englisch, 134 Seiten
Wang / Wölfl / Jiang New Antibody Microarray Tube for Cellular Localization and Signaling Pathways
1. Auflage 2009
ISBN: 978-3-540-74341-5
Verlag: Springer Berlin Heidelberg
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, 134 Seiten
ISBN: 978-3-540-74341-5
Verlag: Springer Berlin Heidelberg
Format: PDF
Kopierschutz: 1 - PDF Watermark
This book introduces a new biochip that incorporates cellular localization and bioinformatics technologies. With the post-genome era, proteomics study for direct analysis of a group of proteins by biochip becomes more and more important. With this book and its 50 tables and figures, the reader can not only learn about new technologies but also about cellular localization, signaling pathways, and protein relationships.
Autoren/Hrsg.
Weitere Infos & Material
1;Chapter 1 Introduction;5
1.1;1.1 Antibody Array Technologies and Applications;5
1.2;1.2 Protein Phosphorylation, Localization and Function;7
1.3;1.3 Interaction Proteomics and Pathway Building;8
2;Chapter 2 Technologies;16
2.1;2.1 Materials;16
2.2;2.2 Methods;18
2.3;2.3 Silver Staining in ArrayTube;21
3;Chapter 3 New Cytoplasmic and Nuclear BMP2- Signaling Pattern in U937 Cells;27
3.1;3.1 3.1 Modulation of Phosphorylation and Localization in BMP2- Treated U937 Cells;27
3.2;3.2 BMP2 Signaling in U937 Cells;36
3.3;3.3 BMP2 Signaling Model in U937 Cells;42
4;Chapter 4 New Cytoplasmic and Nuclear BMP2- Signaling in MCF7 Cells;44
4.1;4.1 Modulation of Phosphorylation and Localization in BMP2- Treated MCF7 Cells;44
4.2;4.2 BMP2 Signaling in MCF7 Cells;52
4.3;4.3 BMP2 Signaling Model in MCF7 Cells;54
5;Chapter 5 New Cytoplasmic and Nuclear STI571 Signaling in K562 Cells;56
5.1;5.1 Modulation of Phosphorylation and Localization in STI571- Treated K562 Cells;56
5.2;5.2 STI571 Signaling in K562 Cells;62
5.3;5.3 STI571 Signaling Model in K562 Cells;65
6;Chapter 6 Positive and Negative Co-localized Rela-tionships in Three Treated Cell Lines;67
6.1;6.1 Positive and Negative Co-localized Distributions;67
6.2;6.2 Possible Relationships;87
7;Chapter 7 Cytoplasmic Signaling in Three Treated Cell Lines;91
7.1;7.1 Cytoplasmic Distribution;91
7.2;7.2 Co-cytoplasmic Signaling;96
8;Chapter 8 Proteomic Silencing in Treated Human Cancer Cell Lines;99
8.1;8.1 Proteomic Silence Distributions;99
8.2;8.2 Possible Relationships;102
9;Chapter 9 Signaling Pathway Setting-up Based on Bioinformatics;104
9.1;9.1 Methods;104
9.2;9.2 Results;110
9.3;9.3 Discussion;121
9.4;9.4 Conclusions;122
10;References;123
