Wassarman Guide to Techniques in Mouse Development, Part A

Mice, Embryos, and Cells
1. Auflage 2010
ISBN: 978-0-08-088778-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 536 Seiten, Web PDF

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.  
This volume is an update of volume 225 of MIE, 'Guide to Techniques in Mouse Development', edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.  During the past 17 years many new technologies or methodologies have appeared for the study of mouse development and this volume comprehensively covers these, including: new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expresion, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, rederivation of animals and use of mouse genomics.

Autoren/Hrsg.

Wassarman, Paul

Inhaltsverzeichnis

1;Front Cover;1
2;Methods in Enzymology: Guide to Techniques in Mouse Development, Part A;4
3;Copyright Page;5
4;Contents;6
5;Contributors;14
6;Preface;20
7;Volume in Series;22
8;Section 1: General Resources
;50
8.1;Chapter 1: A Survey of Internet Resources for Mouse Development;52
8.1.1;1. Introduction;53
8.1.2;2. Mouse Embryo Atlases;54
8.1.3;3. Gene Expression Atlases and Databases;57
8.1.4;4. Locating and Acquiring Mutant Mice and Embryonic Stem Cells;62
8.1.5;5. Discussion;65
8.1.6;6. Further Reading;66
8.1.7;References;66
9;Section 2: Handling Mouse Lines;72
9.1;Chapter 2: Transport of Mouse Lines by Shipment of Live Embryos;74
9.1.1;1. Introduction;75
9.1.2;2. Materials;77
9.1.3;3. Methods;79
9.1.4;4. Results and Discussion;82
9.1.5;References;84
9.2;Chapter 3: Strategies and Considerations for Distributing and Recovering Mouse Lines;86
9.2.1;1. Introduction;87
9.2.2;2. Shipping Live Mice;88
9.2.3;3. Shipping Nonfrozen Embryos or Gametes;91
9.2.4;4. Archiving and Distributing Mouse Lines Through Cryopreservation;95
9.2.5;5. Conclusion;98
9.2.6;Acknowledgment;99
9.2.7;References;99
9.3;Chapter 4: Archiving and Distributing Mouse Lines by Sperm Cryopreservation, IVF, and Embryo Transfer;102
9.3.1;1. Introduction;103
9.3.2;2. Sperm Cryopreservation;104
9.3.3;3. Storing and Shipping Frozen Sperm Samples;108
9.3.4;4. In Vitro Fertilization;110
9.3.5;5. Oviduct Transfer;113
9.3.6;6. Conclusions;116
9.3.7;Acknowledgment;117
9.3.8;References;117
10;Section 3: Gametes and Embryos;120
10.1;Chapter 5: Isolation and Manipulation of Mouse Gametes and Embryos;122
10.1.1;1. Introduction;123
10.1.2;2. Materials, Media, and Solutions;123
10.1.3;3. Collection and Storage Conditions;125
10.1.4;4. Sperm-Egg Interactions;129
10.1.5;5. Analysis of the Status of the Sperm's Acrosome;130
10.1.6;6. Visualization of Gold-Labeled Antibody on Sperm;131
10.1.7;Acknowledgments;132
10.1.8;References;132
10.2;Chapter 6: Cryopreservation of Mouse Gametes and Embryos;134
10.2.1;1. Introduction;135
10.2.2;2. Cryopreservation of Mouse Embryos;135
10.2.3;3. Cryopreservation of Spermatozoa;143
10.2.4;4. Cryopreservation of Oocytes and Ovaries;149
10.2.5;5. Troubleshooting;149
10.2.6;6. Beyond the Methods: Other Considerations;150
10.2.7;7. Summary;152
10.2.8;Acknowledgments;152
10.2.9;References;153
10.3;Chapter 7: Ovarian Follicle Culture Systems for Mammals;156
10.3.1;1. Background;157
10.3.2;2. 2D Culture Systems;161
10.3.3;3. 3D Culture Systems;164
10.3.4;4. Considerations Based on Species Variations;166
10.3.5;5. Conclusions and Future Considerations;167
10.3.6;Acknowledgments;168
10.3.7;References;168
10.4;Chapter 8: Production of Mouse Chimeras by Aggregating Pluripotent Stem Cells with Embryos;172
10.4.1;1. Introduction;173
10.4.2;2. Methods for Producing Chimeras: General Issues;175
10.4.3;3. Preparation of Donor Embryos;177
10.4.4;4. Preparation of Pluripotent Cells;184
10.4.5;5. Making the Aggregates;187
10.4.6;6. Embryo Transfer to Pseudopregnant Females;191
10.4.7;7. Detection of Chimerism and Testing for Germline Transmission;195
10.4.8;8. Discussion;195
10.4.9;References;196
10.5;Chapter 9: Production of Cloned Mice from Somatic Cells, ES Cells, and Frozen Bodies;200
10.5.1;1. Introduction;201
10.5.2;2. Equipment;202
10.5.3;3. Mouse Strains;203
10.5.4;4. Culture Media;203
10.5.5;5. Chemicals;204
10.5.6;6. Protocol;204
10.5.7;References;216
10.6;Chapter 10: Nuclear Transfer in Mouse Oocytes and Embryos;220
10.6.1;1. Introduction;221
10.6.2;2. Equipment and Solutions;221
10.6.3;3. Procedures;224
10.6.4;Acknowledgments;233
10.6.5;References;233
10.7;Chapter 11: Culture of Whole Mouse Embryos at Early Postimplantation to Organogenesis Stages: Developmental Staging and Methods;234
10.7.1;1. Introduction;235
10.7.2;2. Developmental Staging of Postimplantation Mouse Embryos Dissected Between E5.5 and E8.25;237
10.7.3;3. Materials;244
10.7.4;4. Culture Protocols;247
10.7.5;Acknowledgments;251
10.7.6;References;251
10.8;Chapter 12: In Utero and Exo Utero Surgery on Rodent Embryos;254
10.8.1;1. Introduction;255
10.8.2;2. Preparation for Abdominal Surgery;256
10.8.3;3. In Utero Manipulation of Embryos;258
10.8.4;4. Exo Utero Manipulation of Embryos;261
10.8.5;5. Postsurgery;264
10.8.6;6. Experimental Approaches;265
10.8.7;7. Summary;271
10.8.8;References;271
11;Section 4: Fertilization;276
11.1;Chapter 13: Enhancement of IVF in the Mouse by Zona-Drilling;278
11.1.1;1. Introduction;279
11.1.2;2. Materials;282
11.1.3;3. Methods;284
11.1.4;4. Results and Discussion;289
11.1.5;References;298
11.2;Chapter 14: ICSI in the Mouse;300
11.2.1;1. Introduction;301
11.2.2;2. Experimental Procedures;301
11.2.3;Acknowledgments;310
11.2.4;References;310
12;Section 5: ES and iPS Cells;312
12.1;Chapter 15: A Simple Procedure for the Efficient Derivation of Mouse ES Cells;314
12.1.1;1. Introduction;315
12.1.2;2. Methodology/Workflow;318
12.1.3;3. Concluding Remarks and Future Perspective;330
12.1.4;References;331
12.2;Chapter 16: Producing Fully ES Cell-Derived Mice from Eight-Cell Stage Embryo Injections;334
12.2.1;1. Introduction;335
12.2.2;2. Methods;337
12.2.3;3. Summary;342
12.2.4;References;343
12.3;Chapter 17: The Loss-of-Allele Assay for ES Cell Screening and Mouse Genotyping;344
12.3.1;1. Introduction;345
12.3.2;2. Methods;347
12.3.3;References;356
12.4;Chapter 18: Induced Pluripotent Stem Cells;358
12.4.1;1. Generation of iPS Cells;358
12.4.2;2. Expression Vectors for iPS Cell Generation;360
12.4.3;3. Retrovirus Production;363
12.4.4;4. iPS Cell Generation with Retroviral Vectors;364
12.4.5;5. iPS Cell Generation with Proteins and Small Molecules;366
12.4.6;6. iPS Cell Characterization;368
12.4.7;7. Biotechnological and Medical Applications;370
12.4.8;Acknowledgments;370
12.4.9;References;371
13;Section 6: Imaging Mouse Development;376
13.1;Chapter 19: Imaging Mouse Embryonic Development;378
13.1.1;1. Introduction;379
13.1.2;2. Overview of Key Parameters;380
13.1.3;3. Whole Embryo Culture;382
13.1.4;4. Labeling Cells of Interest;387
13.1.5;5. Time-Lapse Imaging of Early Mouse Embryos;389
13.1.6;6. Image Analysis;393
13.1.7;7. Summary;395
13.1.8;References;395
13.2;Chapter 20 Imaging Mouse Development with Confocal Time-Lapse Microscopy;400
13.2.1;1. Introduction;401
13.2.2;2. Materials;404
13.2.3;3. Methods;410
13.2.4;Acknowledgments;424
13.2.5;References;425
13.3;Chapter 21: Ultrasound and Magnetic Resonance Microimaging of Mouse Development;428
13.3.1;1. Introduction;429
13.3.2;2. Ultrasound Biomicroscopy;430
13.3.3;3. Magnetic Resonance Microimaging;434
13.3.4;4. Summary;445
13.3.5;Acknowledgments;445
13.3.6;References;446
14;Section 7: Hematopoiesis;450
14.1;Chapter 22: Use of Transgenic Fluorescent Reporter Mouse Lines to Monitor Hematopoietic and Erythroid Development During Embryogenesis;452
14.1.1;1. Introduction;453
14.1.2;2. Materials;459
14.1.3;3. Methods;462
14.1.4;4. Notes;472
14.1.5;References;472
14.2;Chapter 23: Identification and In Vivo Analysis of Murine Hematopoietic Stem Cells;478
14.2.1;1. Hematopoietic Stem Cells;478
14.2.2;2. Assays of Hematopoietic Stem Cells;480
14.2.3;3. Isolation of Hematopoietic Stem Cells;487
14.2.4;References;491
15;Author Index;498
16;Subject Index;522
17;Color Plate;532