Wellings A Practical Handbook of Preparative HPLC

1. Auflage 2011
ISBN: 978-0-08-045885-4
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 176 Seiten

This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.
This book...
•Provides practical, hands-on advice based on years of experience
•Will help ensure optimal design, equipment and separation results for your particular task
•Presents system layouts from laboratory to process scale
•Will help you to devise or improve record-keeping and documentation systems
·Provides practical, hands-on advice based on years of experience
·Will help ensure optimal design, equipment and separation results for your particular task
·Presents system layouts from laboratory to process scale
·Will help you to devise or improve record-keeping and documentation systems

Autoren/Hrsg.

Wellings, Donald A

Inhaltsverzeichnis

1;Cover;1
2;copyright;5
3;Contents;6
4;front matter;8
5;Preface;8
6;Foreword;10
7;Abbreviations;12
8;body;14
9;1 The history and development of preparative HPLC;14
10;2 Fluid dynamics, mass transport and friction;30
11;3 Modes of chromatographic separation;42
11.1;Particle size;45
11.2;Pore structure, size and surface area;46
11.3;3.1 Normal phase chromatography;48
11.4;3.2 Reversed phase chromatography;50
11.5;3.3 Chiral separations;54
11.5.1;Chirality;55
11.5.2;Optical purity;56
11.5.3;Separation of enantiomers;57
11.6;3.4 Ion exchange chromatography;60
11.7;3.5 Exclusion chromatography;66
11.8;3.6 Affinity chromatography;67
12;4 How to get started;70
12.1;4.1 Packing a column;72
12.1.1;Column packing protocol for column type 1;76
12.1.2;Slurry concentration;76
12.1.3;Slurry and column preparation;77
12.1.4;Column packing protocol for column type 2;78
12.2;4.2 What and where!;79
12.3;4.3 Product recovery;82
12.4;4.4 Productivity;85
13;5 Process development and optimization;90
13.1;5.1 Sample self displacement for purification of a peptide;92
13.1.1;Analytical HPLC;103
13.1.2;Gradient selection;104
13.1.3;Loading study;105
13.1.4;Flow rate optimization;106
13.2;5.2 Boxcar injections for chiral separations;108
14;6 Documentation and record keeping;114
14.1;6.1 Equipment qualification;116
14.2;6.2 Process documentation;118
15;back matter;124
16;Appendices;124
16.1;PROCESS INSTRUCTIONS;126
16.2;CONTENTS;128
16.3;DEVIATION SHEET;130
16.4;OPERATION 1 PREPARATION SECTION;132
16.4.1;1.01 Abbreviations;132
16.4.2;1.02 Target Specifications;132
16.4.3;1.03 Raw Materials;132
16.4.4;1.04 Equipment;134
16.4.5;1.05 Consumables;136
16.4.6;1.06 In-Process Tests;136
16.4.6.1;Analytical HPLC Conditions;136
16.4.6.2;Analytical HPLC Strength Method;136
16.4.7;1.07 Ancillary SOPs;138
16.4.8;1.08 Cleaning;140
16.4.9;1.09 Personnel and Responsibilities;142
16.4.10;1.10 Safety;144
16.4.10.1;Safety Precautions;145
16.5;OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION;148
16.6;OPERATION 3 PURIFICATION;150
16.7;PURIFICATION DEVIATION SHEET;158
16.8;EXAMPLE OF FRACTION RECONCILIATION DOCUMENT;162
16.9;OPERATION 1 SUMMARY;164
17;References;172
17.1;CHAPTER 1;174
17.2;CHAPTER 2;176
17.3;CHAPTER 3;176
17.4;CHAPTER 4;178
17.5;CHAPTER 5;179
17.6;CHAPTER 6;179
18;Index;180