Williamson Genetic Engineering

1. Auflage 2014
ISBN: 978-1-4832-1402-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 180 Seiten, Web PDF

Genetic Engineering 1 is the first of a series containing reviews of particular topics using genetic recombinant DNA techniques. This three-chapter volume describes the construction of libraries of expressed gene sequences, the use of gene-specific probes in antenatal diagnosis, and the expression of isolated genes in cellular and cell-free systems. Chapter 1 presents particular series of steps for the preparation and screening of cDNA clone bank. Chapter 2 discusses the advances in DNA analysis techniques that have profound effects upon the understanding of some genetic diseases and on the ability to reduce the incidence of these diseases by antenatal diagnosis and therapeutic abortion. Chapter 3 considers the experimental systems for studying the expression of isolated eukaryotic genes, concentrating on microinjection into Xenopus oocytes and incubation in cell-free systems in vitro. This book is of great value to genetic engineers, geneticists, and biochemists.

Autoren/Hrsg.

Williamson, Robert

Inhaltsverzeichnis

1;Front Cover;1
2;Genetic Engineering 1;4
3;Copyright Page;5
4;Table of Contents ;10
5;Contributors ;6
6;Preface;8
7;Chapter 1. The preparation and screening of a cDNA clone bank;14
7.1;I Introduction;15
7.2;II The preparation of a cDNA clone bank;18
7.3;III The screening of a cDNA clone bank;44
7.4;IV The future of cDNA cloning;60
7.5;V References;68
8;Chapter 2. DNA analysis and the antenatal diagnosis of hemoglobinopathies;74
8.1;I Introduction;75
8.2;II Globin proteins;77
8.3;III DNA structural analysis;85
8.4;IV Antenatal diagnosis by the direct detection of dysfunctional genes;94
8.5;V Antenatal diagnosis by the indirect detection of dys functional genes—the use of polymorphisms;101
8.6;VI The application and future applications of DNA analysis in antenatal diagnosis;108
8.7;VII Conclusion;112
8.8;VIII Acknowledgements;112
8.9;IX References;113
9;Chapter 3. Expression of cloned genes in cell-free systems and in microinjected Xenopus oocytes;116
9.1;I Introduction;117
9.2;II Transcription of isolated genes by RNA polymerase III;118
9.3;III Transcription of isolated genes by RNA polymerase II;132
9.4;IV Transcription of isolated genes by RNA polymerase I;162
9.5;V Conclusions and prospects;168
9.6;VI Acknowledgements;172
9.7;VII References;172