Wingard / Katchalski-Katzir / Goldstein Immobilized Enzyme Principles

Applied Biochemistry and Bioengineering, Vol. 1
1. Auflage 2014
ISBN: 978-1-4832-1584-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 1, 376 Seiten, Web PDF

Reihe: Applied Biochemistry and Bioengineering

Applied Biochemistry and Bioengineering, Volume 1: Immobilized Enzyme Principles focuses on the utilization of immobilization techniques for the study and application of enzyme catalysts in a variety of potential end-uses. This book emphasizes the preparation of enzyme-support systems, effects caused by the concurrent phenomena of enzyme-catalyzed reaction kinetics and mass transfer resistances, and how these reactions are incorporated into the design of enzyme-catalyzed reactor systems. The magnitude of the perturbation of the apparent kinetic parameters of an immobilized enzyme that could serve in principle as a measure of the effective concentrations of substrate, modifier, or inhibitor at the site of the enzymic reaction is also explained. This volume is recommended for biological scientists and engineers, as well as researchers interested in the biochemical common denominator that causes the interaction of engineering practice and biological sciences for technological development.

Autoren/Hrsg.

Wingard, Lemuel B.

Katchalski-Katzir, Ephraim

Goldstein, Leon

Inhaltsverzeichnis

1;Front Cover;1
2;Immobilized Enzyme Principles;4
3;Copyright Page;5
4;Table of Contents;6
5;List of Contributors;8
6;Introduction to the Series;10
7;Preface;12
8;Chapter 1. Immobilized Enzymes—A Survey;14
8.1;REFERENCES;28
9;Chapter 2. The Chemistry of Enzyme Immobilization;36
9.1;I. INTRODUCTION;36
9.2;II. IMMOBILIZATION OF ENZYMES BY ADSORPTION;38
9.3;III. IMMOBILIZATION OF ENZYMES BY ENTRAPMENT;43
9.4;IV. IMMOBILIZATION OF ENZYMES BY COVALENT CROSS-LINKING WITH Bl- OR MULTIFUNCTIONAL REAGENTS;47
9.5;V. IMMOBILIZATION OF ENZYMES BY COVALENT COUPLING TO POLYMERIC SUPPORTS;54
9.6;VI. CONCLUDING REMARKS;123
9.7;ACKNOWLEDGMENT;123
9.8;REFERENCES;124
10;Chapter 3. Diffusion and Kinetics with Immobilized Enzymes;140
10.1;I. INTRODUCTION;141
10.2;II. FACTORS AFFECTING THE KINETICS OF BOUND ENZYMES;144
10.3;III. HETEROGENEOUS ENZYME KINETICS;152
10.4;IV. EFFECT OF EXTERNAL DIFFUSIONAL LIMITATIONS;157
10.5;V. EFFECT OF INTERNAL DIFFUSIONAL LIMITATIONS;179
10.6;VI. ANALYSIS OF DIFFUSIONAL EFFECTS AND DETERMINATION OF KINETIC PARAMETERS FROM EXPERIMENTAL DATA;193
10.7;VII. DYNAMIC EFFECT OF WEAK ACIDS AND BASES ON HETEROGENEOUS ENZYME KINETICS;201
10.8;VIII. ELECTROSTATIC EFFECTS ON BOUND-ENZYME KINETICS;207
10.9;IX. COIMMOBILIZED MULTIENZYME SYSTEMS;217
10.10;X. PERSPECTIVES;221
10.11;REFERENCES;229
11;Chapter 4. Design and Analysis of Immobilized-Enzyme Flow Reactors;234
11.1;I. INTRODUCTION;235
11.2;II. ENZYME REACTOR TYPES AND THEIR CHOICE;236
11.3;III. IDEALIZED ENZYME REACTOR SYSTEMS;247
11.4;IV. STEADY-STATE ANALYSIS OF MASS TRANSFER AND BIOCHEMICAL REACTION IN ENZYME REACTORS;257
11.5;V. MASS TRANSFER-KINETIC MODELS FOR SPECIALIZED SYSTEMS: TUBULAR REACTORS WITH CATALYTICALLY ACTIVE WALLS/HOLLOW-FIBER REACTORS;284
11.6;VI. NONIDEAL FLUID-FLOW PATTERNS IN ENZYME REACTORS;290
11.7;VII. TRANSIENT ANALYSIS OF IMMOBILIZED-ENZYME REACTORS;294
11.8;VIII. MODELING OF MULTIENZYME REACTOR SYSTEMS;311
11.9;IX. COMBINED REACTION AND SEPARATION PROCESSES;317
11.10;X. PROCESS OPTIMIZATION OF ENZYME REACTORS;320
11.11;XI. COMPARISON OF RELATIVE EFFICIENCIES OF SEVERAL REACTOR DESIGNS;326
11.12;XII. EPILOG;330
11.13;APPENDIX;331
11.14;ACKNOWLEDGMENT;335
11.15;REFERENCES;335
12;Chapter 5.
Industrial Applications of Immobilized Enzymes and Immobilized Microbial Cells;342
12.1;I. INTRODUCTION;342
12.2;II. INDUSTRIAL APPLICATIONS OF IMMOBILIZED ENZYMES;343
12.3;III. APPLICATIONS OF IMMOBILIZED MICROBIAL CELLS;355
12.4;IV. CONCLUSIONS;368
12.5;REFERENCES;369
13;Subject Index;372