Wu / Allis Chromatin and Chromatin Remodeling Enzymes Part C

1. Auflage 2004
ISBN: 978-0-08-049715-0
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 546 Seiten, Web PDF

DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription.
*Genetic assays of chromatin modification and remodeling
*Histone modifying enzymes
*ATP-dependent chromatin remodeling enzymes

Autoren/Hrsg.

Wu, Carl

Allis, C. David

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 377;10
4;Preface;16
5;Volumes in Series;18
6;Section I: Genetic Assays of Chromatin Modification and Remodeling;40
6.1;Chapter 1. Functional Analyses of Chromatin Modifications in Yeast;42
6.2;Chapter 2. Genetic Analysis of Chromatin Remodeling Using Budding Yeast as a Model;94
6.3;Chapter 3. Introduction to Trx-G and Pc-G Genes;100
6.4;Chapter 4. Genetic and Cytological Analysis of Drosophila Chromatin-Remodeling Factors;109
6.5;Chapter 5. Genetic Analysis of H1 Linker Histone Subtypes and Their Functions in Mice;124
7;Section II: Histone Modifying Enzymes;148
7.1;Chapter 6. The Use of Mass Spectrometry for the Analysis of Histone Modifications;150
7.2;Chapter 7. Histone Modification Patterns During Gene Activation;169
7.3;Chapter 8. Identification and Analysis of Native HAT Complexes;193
7.4;Chapter 9. Histone Deacetylases: Purification of the Enzymes, Substrates, and Assay Conditions;206
7.5;Chapter 10. Measurement of Mammalian Histone Deacetylase Activity;219
7.6;Chapter 11. Analysis of Histone Phosphorylation: Coupling Intracellular Signaling to Chromatin Remodeling;236
7.7;Chapter 12. Purification of Histone Methyltransferases from HeLa Cells;252
7.8;Chapter 13. Global Proteomic Analysis of S. cerevisiae (GPS) to Identify Proteins Required for Histone Modifications;266
7.9;Chapter 14. Purification of Sir2 Proteins from Yeast;273
7.10;Chapter 15. Purification and Biochemical Properties of the Drosophila TAC1 Complex;294
7.11;Chapter 16. Isolation and Characterization of CHRASCH, a Polycomb-Containing Silencing Complex;306
7.12;Chapter 17. Biochemical Analysis of Mammalian Polycomb Group Protein Complexes and the Identification of Genetic Elements that Block Polycomb-Mediated Gene Repression;321
8;Section III: ATP-Dependent Chromatin Remodeling Enzymes;336
8.1;Chapter 18. Purification and Functional Analysis of the Mammalian SWI/SNF-Family of Chromatin- Remodeling Complexes;338
8.2;Chapter 19. Isolation and Assay of the RSC Chromatin- Remodeling Complex from Saccharomyces cerevisiae;355
8.3;Chapter 20. DNA Translocation and Nucleosome Remodeling Assays by the RSC Chromatin Remodeling Complex;361
8.4;Chapter 21. A Nucleosome Sliding Assay for Chromatin Remodeling Factors;383
8.5;Chapter 22. Methods for Analysis of Nucleosome Sliding by Drosophila NURF;392
8.6;Chapter 23. Methods for Preparation and Assays for Xenopus ISWI Complexes;403
8.7;Chapter 24. Functional Analysis of ISWI Complexes in Mammalian Cells;415
8.8;Chapter 25. Preparation and Assays for Mammalian ISWI Complexes;428
8.9;Chapter 26. Preparation and Analysis of the INO80 Complex;440
8.10;Chapter 27. Assay of Z-DNA Induction by Chromatin Remodeling Factors;451
8.11;Chapter 28. Immuno-Depletion and Purification Strategies to Study Chromatin-Remodeling Factors In Vitro;460
9;Section IV: Transcription and Other Transactions on Chromatin Templates;482
9.1;Chapter 29. Transcription Through the Nucleosome by mRNA-producing RNA Polymerases;484
9.2;Chapter 30. Reconstitution and Transcriptional Analysis of Chromatin In Vitro;499
9.3;Chapter 31. Techniques Used to Study Transcription on Chromatin Templates;513
9.4;Chapter 32. Analysis of DNA Repair on Nucleosome Templates;538
10;Author Index;548
11;Subject Index;574