Wu / Cheng Plant Antiviral Resistance

Methods and Protocols
Erscheinungsjahr 2026
ISBN: 978-1-0716-5300-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 182 Seiten

Reihe: Springer Protocols

This volume aims to provide a comprehensive collection of modern experimental techniques, protocols, and analytical tools for studying plant antiviral resistance.  Chapters detail viral infection, plant immunity, advanced methodologies for high-throughput screening, omics-based profiling, and cutting-edge proximity tagging technology applications.  Written in the highly successful   series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative,   is a valuable resource for researchers and scientists who want to learn more about this important and developing field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Wu, Guanwei

Cheng, Xiaofei

Inhaltsverzeichnis

Development of Tobamovirus-Resistant Tomato Plants by CRISPR-Cas9-Mediated Knockout of Susceptibility Genes.- Silencing host factors with hairpin RNA for virus resistance.- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana Using a Meristem-Invading Virus Vector.- Pepper veinal mottle virus (PVMV)-based vector for expressing pathogen-derived resistance in pepper.- Construction of Attenuated Viruses via Changing the UpA Dinucleotide Frequency.- Mild strain cross-protection in passion fruit.- A TurboID-based protocol for efficient interacting proteins identification using proximity tagging technology.- Protocol for Exogenous Jasmonate Treatment and Quantitative Assessment of Plant Virus Infection.- Exogenous melatonin application via foliar spray and root irrigation for plant virus control.- Phosphatidic acid application to enhance plant virus resistance.- Virus elimination by thermotherapy combined with meristem culture.- Combining chemical treatment with shoot tip culture for plant virus eradication in fruit trees.- Design Artificial Resistance Using Plant Elicitor Peptides.- Virus-induced NLR-silencing library-based screening to identify antiviral NLR genes.- VIGS-based screening for NLR genes regulating viral effector-induced hypersensitive response.- High-Throughput Screening of Antiviral Agents Using the Fluorescence-Based Local Lesion Inspection Assay (FLLIA).- Enhancing Plant Antiviral Resistance with Microbial Immunomodulators: A Half-Leaf Spot Screening Method.- Screening and identification of antiviral chemical components from medical plants.- Development of a reverse genetics system for rice tiller inhibition virus.- Methods for Autophagy Detection by Fluorescence Microscopy.