Yokomori / Shirahige Cohesin and Condensin

Methods and Protocols
1. Auflage 2017
ISBN: 978-1-4939-6545-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1515, 276 Seiten, eBook

Reihe: Methods in Molecular Biology

The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Yokomori, Kyoko

Shirahige, Katsuhiko

Inhaltsverzeichnis

A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts.- An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex.- Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl.- Detection of Cohesin SUMOylation In Vivo.- Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.-  Protein and Chromosome Analysis in Mammalian Meiocytes.- Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex.- Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq.- A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.- Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing.- Zebrafish as a Model to Study Cohesin and Cohesinopathies.- Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.- Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins.- The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair.- A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells.- ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells.