Microbial Steroids

Methods and Protocols
2. Auflage 2023
ISBN: 978-1-07-163384-7
Verlag: Springer US

Buch, Englisch, Band 2704, 355 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 896 g

Reihe: Methods in Molecular Biology

This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Barredo, José-Luis

Barreiro, Carlos

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

Part I: General Overview



1. Current Trends and Perspectives in Microbial Bioconversions of Steroids

Marina V. Donova



Part II: Microbial Screening and Genetic Manipulation



2. Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida

Alejandro Chamizo-Ampudia, Luis Getino, José M. Luengo, and Elias R. Olivera



3. Selection of Biodegrading Phytosterol Strains

María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal



4. Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas

José M. Luengo and Elias R. Olivera



5. Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination

Shikui Song and Zhengding Su



Part III: Genetic and Biochemical Analyses



6. RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics

Antonio Rodríguez-García, Alberto Sola-Landa, and Carlos Barreiro



7. Bidimensional Analyses of the Intra- and Extra-Cellular Proteomes of Steroid-Producer Mycobacteria

Carlos Barreiro and Ana M. Ibáñez



8. Fungal Sterol Analyses by Gas Chromatography - Mass Spectrometry Using Different Derivatives

Aurélie Mossion, Isabelle Ourliac-Garnier, and Gaetane Wielgosz-Collin



9. Cholesterol Assay Based on Recombinant Cholesterol Oxidase, ABTS, and Horseradish Peroxidase

Alexey V. Sviridov, Mikhail V. Karpov, Victoria V. Fokina, and Marina V. Donova



10. Measuring the Interaction of Sterols and Steroids with Proteins by Microscale Thermophoresis

Ola El Atab and Roger Schneiter



Part IV: Fermentation and Biocatalysis for Steroids Production



11. Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products

Simone Balzer Le, Anna Nordborg, Kjell Domaas Josefsen, Silje Malene Olsen, and Håvard Sletta



12. ß-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors

Marco P.C. Marques, Jorge Aranda Hernandez, and Pedro Fernandes



13. Biocatalysis of Steroids by Mycobacterium sp. in Aqueous and Organic Media

Carla C.C.R. de Carvalho and Pedro Fernandes



14. Scale-Up of Phytosterols Bioconversion into Androstenedione

Sonia Martínez-Cámara, Manuel de la Torre, José-Lu