Basu PCR Primer Design

Third Auflage 2022
ISBN: 978-1-0716-1799-1
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2392, 276 Seiten, eBook

Reihe: Methods in Molecular Biology

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR,  in silico  PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful  Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible,  PCR Primer Design, Third Edition  aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Basu, Chhandak

Inhaltsverzeichnis

The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species.- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques.- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.- Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products.- Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences.- Designing PCR primers for the amplification-refractory mutation system.- Validation of circular RNAs by PCR.- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana.- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units.- Multiplex PCR Design for Scalable Resequencing.-  Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR).- qPrimerDB: A powerful and user-friendly database for qPCR primer design.- PCR primer design for the rapidly evolving SARS-CoV-2 genome.- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example.- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis.- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate methylated oligonucleotides as a novel primer for PCR and RT-PCR.