Buch, Englisch, Band 2392, 276 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 751 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 2392, 276 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 751 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-1798-4
Verlag: Springer US
This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
SECTION I. Primer design for genotyping
- The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species
M. Delghandi, M.P. Delghandi, S. Goddard
2. Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.
Amita Pandey, Binu Bhat, Madan L. Aggarwal, Girdhar K. Pandey
3. Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques
Luis Antonio Tortajada-Genaro
4. Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.
Suji George
5. Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
6. Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences
Lidia Skuza
7. Designing PCR primers for the amplification-refractory mutation system
Majid Komijani, Khashayar Shahin, Esam Ibraheem Azhar, Mohammad Bahram
SECTION II. Primer design for genome-wide identification of specific regions
8. Validation of circular RNAs by PCR
Aniruddha Das, Debojyoti Das, and Amaresh C. Panda
9. Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana
Pinky Dhatterwal, Sandhya Mehrotra, and Rajesh Mehrotra
SECTION III. Primer design for multiplex PCR. Multiplex
10. PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units
Raquel Cuartas, Teresa M. Coque, Fernando de la Cruz, M. Pilar Garcillán-Barcia
11. Multiplex PCR Design for Scalable Resequencing
Darren Korbie, Matt Trau
SECTION IV. Primer design for qPCR
12. Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR)
Poonam Kanwar, Soma Ghosh, Sibaji K. Sanyal, Girdhar K. Pandey
13. qPrimerDB: A powerful and user-friendly database for qPCR primer design
Wei Chang, Yue Niu, Mengna Yu, Tian Li, Jiana Li, Kun Lu
SECTION V. Primer design for identification of plant and animal viruses
14. PCR primer design for the rapidly evolving SARS-CoV-2 genome
Jiangyu Li, Dongsheng Zhao, Haoyang Cai and Wubin Qu
15. &n
