Berardi Extracellular Matrix for Tissue Engineering and Biomaterials

1. Auflage 2018
ISBN: 978-3-319-77023-9
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 218 Seiten

Reihe: Stem Cell Biology and Regenerative Medicine

This volume provides a state-of-art-report on the new methodologies in tissue engineering and developments in the biomaterials field based on the extracellular matrix-relevant discovery. Extracellular Matrix for Tissue Engineering and Biomaterials opens with an overview of the latest extracellular matrix research and in Part I, focuses on its biology and its role on cell behavior and cell fate relevant for the design of biomimetic surfaces. Part II details issues regarding  the strategies currently applied in the research of biologically inspired materials and material systems for the replacement, repair and regeneration of tissues and organs. Part III presents the latest development methods applying knowledge from biology towards nanotechnology, to promote the restoration of the functionality of a living tissue. The book ranges from fundamental biology associated with tissue regeneration for the development of biomimetic approaches to controlling tissue formation, cell function, differentiation and angiogenesis using factors involved in normal tissue development and function. With the breadth and depth of the coverage of this topic, this book will serve as a valuable reference for anyone working in tissue engineering or biomaterials - from scientists, chemists and biologists through physicists, bioengineers and clinicians.

Anna C Berardi currently serves as the head of the Stem Cells Laboratory, and she is also the Head of the Research and Development Laboratory in the U.O.C. of Immunohematology, Transfusion Medicine and Hematology Laboratory at the Pescara Civil Hospital. She completed her Ph.D. in Dr. David Scadden's laboratory at the Research Laboratory Hematology/Oncology of New England Deaconess Hospital, Harvard Medical School in Boston, MA. She did post-doc work at Dr. William Vainchenker's Laboratory at the Research of Hematology and Stem Cells, Institut Gustave-Roussy, Villejuif, Paris, and also worked at the Laboratory of Molecular Citology in the Center of Advanced Biotechnologies, Genoa. She was P.I. at the Laboratory of Haematology-Oncology, National Health Institute, Rome before serving as Head of the research laboratory on Stem Cells at the Onco-Haematology Department at Clinic Institute Humanitas, Rozzano, Milan and Head of the 'Stem Cells' Laboratory Bambino Gesù, Children Hospital, Rome. She was Visiting Professor at the Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD. Dr. Berardi has devoted her career largely to biomedical research towards hematopoietic stem cells biology, including tissue engineering and regenerative medicine and stem cells technology. Through cutting-edge technologies and broadly collaborative approaches, she is also committed to training the new generation of stem cell biologists and to building a strong, unique and competitive stem cell research program that will ultimately benefit patients.

Autoren/Hrsg.

Berardi, Anna C.

Inhaltsverzeichnis

1;Preface;6
2;Contents;7
3;Contributors;9
4;Abbreviations;11
5;Extracellular Matrix;12
6;1 The Extracellular Matrix, Growth Factors and Morphogens in Biomaterial Design and Tissue Engineering;13
6.1;Abstract;13
6.2;Introduction;14
6.3;Key Molecular ECM Components;14
6.4;Prominent Role of the Proteoglycans and Glycosaminoglycans;16
6.5;Fibrous Proteins and Adhesive Glycoproteins;17
6.6;Growth Factors;18
6.7;Protease Activity and Role of Proteolytic Enzyme in ECM;21
6.8;Extracellular Vesicles in the ECM Structure/Organization;22
6.9;Importance of ECM–Integrin Interactions;23
6.10;Mechanical Signals Inside the ECM;25
6.11;Morphogenesis;26
6.12;Morphogenesis of 3D Tissue Architecture in Vivo: Folds, Tubes, and Branches;27
6.13;Growth Factors and Morphogens for Tissue Engineering;28
6.14;Growth Factor and Morphogen Delivery Through Engineered ECM;29
6.15;Engineering Growth Factors and Morphogens for Interaction with Exogenous Biomaterials and for Delivery Through the Native ECM;30
6.16;References;30
7;2 ECM Hydrogels for Regenerative Medicine;37
7.1;Abstract;37
7.2;Historical Development of ECM-Derived Materials;38
7.2.1;Biochemical Content;39
7.2.2;Bioinductive Properties;40
7.2.2.1;Antimicrobial Properties;40
7.2.2.2;Chemoattraction;40
7.2.2.3;Macrophage Polarization;41
7.2.3;Types of ECM-Derived Materials;42
7.3;ECM Hydrogel Formation;43
7.3.1;Methods for ECM Hydrogel Production;44
7.3.2;ECM Hydrogel Characterization;45
7.3.2.1;Biocompatibility;45
7.3.2.2;Biochemical Composition;46
7.3.2.3;Gelation Kinetics and Mechanical Properties;46
7.3.2.4;Gel Topology;48
7.4;Tissue-Specific Hydrogels;48
7.4.1;Heart;49
7.4.2;Fat;51
7.4.3;Skin;53
7.4.4;Liver;53
7.4.5;Skeletal Muscle;54
7.4.6;Central Nervous System;55
7.4.7;Cartilage;56
7.4.8;Tendon and Ligament;57
7.4.9;Intervertebral Disk;58
7.4.10;Others;59
7.5;Hybrid Hydrogels;60
7.6;Future Directions;61
7.7;References;62
8;3 Biologically Relevant Laminins in Regenerative Medicine;69
8.1;Abstract;69
8.2;Introduction;70
8.3;Cell Niche and Extracellular Matrix;72
8.3.1;Expanding Cells in Vitro: Future of Regenerative Medicine;72
8.3.2;Three Pillars of Cell Niche: Growth Factors, Cell–Cell Contacts, and Niche-Specific Extracellular Matrix;73
8.3.3;Laminins: Sixteen Niche-Specific Extracellular Matrix Molecules with Unique Biological Function;74
8.4;Laminins: Molecular Aspects and Cell Signaling;74
8.4.1;Laminins: Chains and Trimers;75
8.4.2;Molecular Interactions of Laminins;78
8.4.2.1;Interactions with Cell Receptors and Co-Signaling;78
8.4.2.2;Extracellular Matrix Interactions;79
8.4.2.3;Mechanotransduction;79
8.4.3;Proteolytically Degraded Forms of Laminins;80
8.4.4;Possible Antagonistic Functions of Related Laminins;80
8.5;Biologically Relevant Laminins for In Vitro Cell and Organoid Cultures;81
8.5.1;Human and Mouse Embryonic Stem Cells;81
8.5.2;Bone Marrow-Derived Hematopoietic Stem Cells;83
8.5.3;Insulin-Producing Pancreatic Islets;83
8.5.4;Neurobiology;84
8.6;Evaluation Criteria in Developing Cell Culture System;84
8.7;Future Challenges;85
8.8;Summary;86
8.9;Acknowledgements;87
8.10;References;87
9;4 Extracellular Matrix: Immunity and Inflammation;93
9.1;Abstract;93
9.2;The Extracellular Matrix;93
9.2.1;Matrix Metalloproteinases (MMPs);95
9.2.2;Versican;97
9.2.3;Hyaluronan;99
9.2.4;Thrombospondins;99
9.3;Cells;100
9.4;Inflammation;101
9.4.1;Metalloproteinases and Inflammation;102
9.4.2;Versican and Inflammation;104
9.4.3;Hyaluronan and Inflammation;105
9.4.4;Thrombospondins and Inflammation;106
9.5;Immunity;108
9.5.1;Metalloproteinases and Immunity;109
9.5.2;Versican and Immunity;111
9.5.3;Hyaluronan and Immunity;112
9.5.4;Thrombospondins and Immunity;113
9.6;References;114
10;Material Inspired from Nature;120
11;5 Biologically Inspired Materials in Tissue Engineering;121
11.1;Abstract;121
11.2;Functional Fibrous Scaffolds for Tissue Engineering Applications;122
11.3;3D Drug Releasing Scaffolds;128
11.4;Fibrinogen-Based Scaffolds;135
11.5;References;143
12;Nanotechnologies and Biomimetic;156
13;6 Advances in Nanotechnologies for the Fabrication of Silk Fibroin-Based Scaffolds for Tissue Regeneration;157
13.1;Abstract;157
13.2;Conclusions;164
13.3;References;164
14;7 Nanoscale Architecture for Controlling Cellular Mechanoresponse in Musculoskeletal Tissues;167
14.1;Abstract;167
14.2;Nanometric Architecture;168
14.3;Nanoscale Engineering;168
14.4;Tensegrity;169
14.5;Architecture and Prestress;169
14.6;Tensegrity and Mechanochemical Transduction;171
14.7;Hormesis;173
14.8;Bone and Osteoinduction;176
14.9;ECM and Tendons;179
14.10;Proprioception: Muscle, Tendon, and Ligament;180
14.11;Nanoscale Biotechnology;185
14.12;Conclusions;189
14.13;References;190
15;8 Modular Tissue Engineering: An Artificial Extracellular Matrix to Address and Stimulate Regeneration/Differentiation;196
15.1;Abstract;196
15.2;The “Top-Down” or “Bottom-Up” Approach: Which Is the Best Choice?;196
15.3;Biomaterials for Scaffold Fabrication;199
15.4;3D Scaffold Fabrication: The Organic Solvent Can Be Replaced by Supercritical Fluid;200
15.5;Microdevices and Microcapsules Fabrication: A Challenge for Supercritical Fluid Technology;204
15.6;Micro-/Nanocarriers as Functional Components for a Bottom-Up Approach to Bioengineered Scaffold;205
15.7;Conclusions and Perspectives;210
15.8;References;211
16;Index;216