RNA Methodologies

A Laboratory Guide for Isolation and Characterization
6. Auflage 2022
ISBN: 978-0-323-90221-2
Verlag: William Andrew Publishing

Buch, Englisch, 920 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1540 g

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area.

This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.

Zielgruppe

<p>Researchers and investigators in molecular biology and biochemistry, biomedicine, and related fields </p> <p>Graduate and postdoctoral students in molecular biology, biomedicine, and related life science programs</p>

Autoren/Hrsg.

Farrell Jr., Robert E.

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. RNA and the cellular biochemistry revisited

2. Creating a ribonuclease-free environment 000
3. Stringency: conditions that influence nucleic acid structure
4. Ribonucleic acid isolation strategies
5. Isolation of polyadenylated RNA
6. The truth about tissues
7. Going green: RNA and the molecular biology of plants
8. Quality control for RNA preparations
9. cDNA: a permanent biochemical record of the cell
10. RT-PCR: a science and an art form
11. Quantitative polymerase chain reaction techniques
12. miRNA and other noncoding RNAs
13. RNA interference and gene editing
14. Electrophoresis of RNA
15. Photodocumentation and image analysis
16. Northern analysis
17. Nucleic acid probe technology
18. Quantification of specific mRNAs by nuclease protection
19. Analysis of nuclear RNA
20. RNA in situ hybridization
21. Array analysis of gene expression
22. Subtractive and nonsubtractive methods for the analysis of gene expression
23. Transcriptomes and bioinformatics
24. RNA-seq: the premier transcriptomics tool
25. RNA biomarker discovery and validation
26. Functional genomics strategies
27. A few RNA success stories

Epilogue
Appendix A: Maintaining complete and accurate records

Appendix B: Converting mass to moles

Appendix C: Removal of DNA from an RNA sample

Appendix D: Removal of RNA from a DNA sample

Appendix E: Electrophoresis: principles, parameters, and safety

Appendix F: Disposal of ethidium bromide and SYBR Green solutions

Appendix G: Deionization of formamide, formaldehyde, and glyoxal

Appendix H: Silanizing centrifuge tubes and glassware

Appendix I: Centrifugation as a mainstream tool for the molecular biologist

Appendix J: Dot blot analysis

Appendix K: Useful stock solutions for the molecular biologist

Appendix L: Genomes and proteomes

Appendix M: Common SI prefixes

Appendix N: Common abbreviations

Appendix O: Select suppliers of equipment, reagents, and services

Appendix P: Trademark citations

Glossary

Über Autor(innen)

Farrell Jr., Robert E.
Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.