Hummel Ancient DNA Typing

Methods, Strategies and Applications
Erscheinungsjahr 2013
ISBN: 978-3-662-05050-7
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 298 Seiten, eBook

Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering. The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.

Zielgruppe

Research

Autoren/Hrsg.

Hummel, Susanne

Inhaltsverzeichnis

1 Introduction.- 2 DNA markers.- 3 aDNA extraction.- 4 PCR.- 5 Analysis of PCR products.- 6 Authenticity of results.- 7 Applications.- 8 Protocols.- I Laboratory equipment.- I.1 Pre-PCR laboratory equipment.- I.2 PCR laboratory.- I.3 Post-PCR laboratory.- I.4 Photographic documentation.- II PCR trouble shooting.- III Internet sites of interest.