Buch, Englisch, 370 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 5796 g
Applications, Methods, Instrumentation
Buch, Englisch, 370 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 5796 g
Reihe: Springer Series on Fluorescence
ISBN: 978-3-319-35429-3
Verlag: Springer International Publishing
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Modern TCSPC electronics - Principles and Acquisition Modes
Michael Wahl
Single-photon counting detectors for the visible range between 300 nm and 1000 nm
Andreas Bülter
Single-photon detectors for infrared wavelengths in the range 1 to 1.7 µm
Gerald S. Buller and Robert J. Collins
Modern pulsed diode laser sources for time-correlated photon counting
Kristian Lauritsen, Sina Riecke#, Andreas Bülter, Thomas Schönau
Advanced FCS: an Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual Focus FCS
Thomas Dertinger and Steffen Rüttinger
Lifetime-weighted FCS and 2D FLCS: Advanced application of time-tagged TCSPC
Kunihiko Ishii, Takuhiro Otosu, and Tahei Tahara
MFD-PIE and PIE-FI: Ways to extract more information with TCSPC
Anders Barth, Lena Voith von Voithenberg, Don C. Lamb
Photon Antibunching in Single Molecule Fluorescence Spectroscopy
Kristin S. Grußmayer and Dirk-Peter Herten
FLIM Strategies for Intracellular Sensing: Fluorescence Lifetime Imaging as a Tool to Quantify Analytes of Interest
Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte
Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue
Rafal Fudala, Ryan M. Rich, Joe Kimball, Igna
cy Gryczynski, Sangram Raut, Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, Zygmunt GryczynskiPattern based linear un-mixing for efficient and reliable analysis of multi-component TCSPC-data
Ingo Gregor and Matthias PattingMetal-Induced Energy Transfer
Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik
The importance of photon arrival times in STED microscopy
Giuseppe Vicidomini
Single color centers in diamond as single photon sources and quantum sensors
Boris Naydenov and Fedor Jelezko
Photon counting and timing in quantum optics experiments
Andreas Ahlrichs, Benjamin Sprenger, Oliver Benson
Photon counting in diffuse optical imaging
Dirk Grosenick
