Li Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

Erscheinungsjahr 2010
ISBN: 978-1-4200-2745-7
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 528 Seiten

Reihe: Chromatographic Science Series

The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information.

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays.

Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.

Zielgruppe

Chemical engineers, biolomedical researchers, and materials scientists.

Autoren/Hrsg.

Li, Paul C.H.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe

Inhaltsverzeichnis

Introduction

Micromachining Methods
Micromachining of Silicon

Micromachining of Glass

Micromachining of Fused Quartz (or Fused Silica)

Micromachining of Polymeric Chips
Metal Patterning

World-to-Chip Interface

Microfluidic Flow
Liquid Pumping Methods

Microfluidic Flow Control

Sample Introduction
Electrokinetic Injection

Hydrodynamic Injection

Other Sample Injection Methods

Sample Pre-concentration
Sample Stacking

Extraction

Porous Membrane

Other Pre-concentration Methods

Separation
Gas Chromatography (GC)

Capillary Electrophoresis (CE)

Chromatographic Separations

Coupled Separations

Detection Methods
Optical Detection Methods

Electrochemical (EC) Detection

Mass Spectrometry (MS)

Other Detection Methods

Applications to Cellular/Particle Analysis
Retention of Cells and Particles

Studies of Cells in a Flow

Other Cell Operations

Applications to Nucleic Acids Analysis
Nucleic Acids Extraction and Purification

Nucleic Acids Amplification

DNA Hybridization

Other Nucleic Acid Applications

Applications to Protein Analysis
Immunoassay

Protein Separation

Enzymatic Assays

Appendix

Problem Sets

Introduction

Micromachining

Microfluidic Flow

Sample Introduction

Sample Preconcentration

Separation

Detection

Cellular Analysis

Nucleic Acid Analysis

Protein Analysis

References

Glossary