E-Book, Englisch, 184 Seiten
Matsudaira A Practical Guide to Protein and Peptide Purification for Microsequencing
2. Auflage 2012
ISBN: 978-0-08-092461-8
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark
E-Book, Englisch, 184 Seiten
ISBN: 978-0-08-092461-8
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark
Why a Second Edition?The Second Edition provides practical answers to the general question, 'How can I obtain useful sequence information from my protein or peptide?' rather than the more specific question asked in the first edition, 'How can I obtain the N-terminal sequence?' Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing