E-Book, Englisch, 317 Seiten
Pawley / Schatten Biological Low-Voltage Scanning Electron Microscopy
1. Auflage 2007
ISBN: 978-0-387-72972-5
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, 317 Seiten
ISBN: 978-0-387-72972-5
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark
Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.
Autoren/Hrsg.
Weitere Infos & Material
1;Preface;5
2;Contents;7
3;List of Contributors;9
4;The Early Development of the Scanning Electron Microscope;11
5;LVSEM for Biology;36
6;The Aberration-Corrected SEM;116
7;Noise and Its Effects on the Low-Voltage SEM;137
8;High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy ( HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols;153
9;Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF- TEM and HVEM;178
10;Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live- Time Experiments in the SEM;204
11;Cryo-SEM of Chemically Fixed Animal Cells;222
12;High-Resolution and Low-Voltage SEM of Plant Cells;236
13;High-Resolution Cryoscanning Electron Microscopy of Biological Samples;252
14;Developments in Instrumentation for Microanalysis in Low- Voltage Scanning Electron Microscopy;270
15;Index;311
