Buch, Englisch, 455 Seiten, Previously published in hardcover, Format (B × H): 160 mm x 240 mm, Gewicht: 760 g
Principles and Applications
Buch, Englisch, 455 Seiten, Previously published in hardcover, Format (B × H): 160 mm x 240 mm, Gewicht: 760 g
ISBN: 978-90-481-7100-2
Verlag: Springer Netherlands
When considering the biological significance and industrial and medical applications of biopolymers, it is crucial to know details of their secondary structure, dynamics and assembly. Solid state NMR spectroscopy has proved to be the most suitable and unrivaled means for investigations of biopolymers. Special efforts have been made to include the historical and chronological consequences of a variety of applications and the dynamic aspects of the biopolymer system. In particular, the authors emphasise how important it is to record the simplest DD-MAS as a mean of locating very flexible portions of membrane proteins and membrane associated peptides. The authors also demonstrate that dynamic features of membrane proteins with a timescale of fast and intermediate fluctuation motions can be revealed easily by specific suppression of peaks.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
- Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Werkstoffkunde, Materialwissenschaft: Forschungsmethoden
- Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie
- Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Werkstoffprüfung
- Naturwissenschaften Chemie Physikalische Chemie
- Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Materialwissenschaft: Verbundwerkstoffe
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Technologie der Kunststoffe und Polymere
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie
- Naturwissenschaften Physik Thermodynamik Festkörperphysik, Kondensierte Materie
- Naturwissenschaften Chemie Organische Chemie Polymerchemie
- Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Materialwissenschaft: Polymerwerkstoffe
- Naturwissenschaften Chemie Anorganische Chemie Festkörperchemie
Weitere Infos & Material
Part I Principles
1. Introduction
2. Solid state NMR approach
2.1. CP-MAS and DD-MAS NMR
2.2. Quadrupolar nuclei
3. Brief outline of NMR parameters
3.1. Chemical shifts
3.2. Relaxation parameters
3.3. Dynamics-dependent suppression of peaks
4. Multinuclear approaches
4.1. 31P NMR
4.2. 2H NMR
4.3. 17O NMR
5. Experimental strategies
5.1. Isotope enrichment (labeling)
5.2. Assignment of peaks
5.3. Ultra high-field and ultra high-speed MAS NMR spectroscopy
6. NMR constraints for structural determination
6.1. Orientational constraint
6.2. Interatomic distance
6.3. Torsion angles
6.4. Conformation-dependent 13C chemical shifts
7. Dynamics
7.1. Fast motions with motional frequency >106 Hz
7.2. Intermediate or slow motions with frequency between 106 and 103 Hz
7.3. Very slow motions with frequency < 103 Hz
Part II Applications
8. Hydrogen bonded systems
8.1. Hydrogen bond shifts
8.2. 2H quadrupolar coupling constant
9. Fibrous proteins
9.1. Collagen fibrils
9.2. Elastin
9.3. Cerial proteins
9.4. Silk fibroin
9.5. Keratin
9.6. Bacteriophage coat protein
10. Polysaccharides
10.1. Distinction of polymorphs
10.2. Network structure, dynamics and gelation mechanism
11. Polypeptides as new materials
11.1. Liquid crystalline polypeptides
11.2. Blend system
12. Globular proteins
12.1. (Almost) complete assignment of 13C NMR spectra of globular proteins
12.2. 3D structure: ?-spectrin SH3 domain
12.3. Ligand-binding to globular protein
13. Membrane protein I: dynamic picture
13.1. Bacteriorhodopsin
13.2. Phoborhodopsin and its cognitive transducer
13.3. Diacylgycerol kinase
14. Membrane proteins II: 3D structure
14.1. 3D structure of mechanically aligned membrane proteins
14.2. Secondary structure based on distance constraints
15. Biologically active membrane-associated peptides
15.1. Channel-forrming peptides
15.2. Antimicrobial peptides
15.3. Opioid peptides
15.4. Fusion peptides
15.5. Membrane model system
17. Amyloid and related biomolecules
17.1. Amyloid ?-peptide
17.2. Calcitonin (CT)
