Protocols for Oligonucleotide Conjugates

Synthesis and Analytical Techniques
1994. Auflage 1993
ISBN: 978-0-89603-252-1
Verlag: Humana Press

Buch, Englisch, Band 26, 377 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 576 g

Reihe: Methods in Molecular Biology

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

Zielgruppe

Research

Autoren/Hrsg.

Agrawal, Sudhir

Fachgebiete

• Naturwissenschaften Chemie Organische Chemie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Pharmazeutische Technologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Protecting Groups in Oligonucleotide Synthesis.- Incorporation of Modified Bases into Oligonucleotides.- Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups.- Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups.- Postsynthesis Functionalization of Oligonucleotides.- Oligonucleotide-Enzyme Conjugates.- Oligonucleotides Containing Degenerate Bases.- Synthesis of [15N]-Labeled DNA Fragments.- Analysis and Purification of Synthetic Oligonucleotides by High-Performance Liquid Chromatography.- Sequence Analysis of Oligodeoxyribonucleotides.- Gel-Capillary Electrophoresis Analysis of Oligonucleotides.- Nuclear Magnetic Resonance Studies of Oligonucleotides.- Mass Spectrometry of Nucleotides and Oligonucleotides.- Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions.