Buch, Englisch, Band 645, 230 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 482 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 645, 230 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 482 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-689-0
Verlag: Humana Press
Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Table of Contents
Preface
Contributors
1. The Role of Inositol and the Principals of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells
Christopher J. Barker and Per-Olof Berggren
2. HPLC Separation of Inositol Polyphosphates
Christopher J. Barker, Christopher Illies, and Per-Olof Berggren
3. Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells
James C. Otto and John D. York
4. A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate
Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine
5. Synthesis of InsP7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1)
Cristina Azevedo, Adam Burton, Matthew Bennett, Sara Maria Nancy Onnebo, and Adolfo Saiardi
6. Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP7)
J. Kent Werner, Jr., Traci Speed, and Rashna Bhandari
7. Synthesis and Non-Radioactive Micro-Analysis of Diphosphoinositol Phosphates by HPLC with Post-Column Complexometry
Hongying Lin, Karsten Lindner, and Georg W. Mayr
8. Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio
Andreas Nagel, Christopher J. Barker, Per-Olof Berggren, and Christopher Illies
9. Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference
Akihiro Mizutani, Kastuhiro Kawaai, Chihiro Hisatsune, Hideaki Ando, Takayuki Michikawa, and Katsuhiko Mikoshiba
10. Phosphoinositide-Specific Phospholipase C b1 Signal Transduction in the Nucleus
Roberta Fiume, Gabriella Teti, Irene Faenza, and Lucio Cocco
11. Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides
Nullin Divecha
12. Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC
Frank T. Cooke
13. Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry
Trevor R. Pettitt
14. Imaging Phosphoinositide Dynamics in Living Cells
Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm