Baumann G-Quadruplex DNA

Methods and Protocols
Erscheinungsjahr 2009
ISBN: 978-1-59745-363-9
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 608, 276 Seiten, eBook

Reihe: Methods in Molecular Biology

Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In "G-Quadruplex DNA: Methods and Protocols", experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, "G-Quadruplex DNA: Methods and Protocols "promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Baumann, Peter

Inhaltsverzeichnis

G-Quadruplexes: From Guanine Gels to Chemotherapeutics.- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes.- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential.- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis.- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay.- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy.- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures.- 2-Aminopurine as a Probe for Quadruplex Loop Structures.- Assessing DNA Structures with 125I Radioprobing.- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques.- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments.- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC.- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography.- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes.- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.