Buch, Englisch, Band 1222, 285 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 569 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1222, 285 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 569 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-5441-4
Verlag: Springer
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie
- Naturwissenschaften Biowissenschaften Molekularbiologie
- Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
- Naturwissenschaften Biowissenschaften Zellbiologie
Weitere Infos & Material
1. Nuclear Transfer in the Mouse
Vincent Brochard and Zichuan Liu
2. Nuclear Transfer in the Rabbit
Nathalie Daniel and Patrick Chesné
3. Nuclear Transfer in Ruminants
Joon-Hee Lee and Walid E. Maalouf
4. Nuclear Transfer and Transgenesis in the Pig
Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, and Eckhard Wolf
5. Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation
Huseyin Sumer and Paul J. Verma
6. Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte
Jerome Jullien
7. Assessing the Quality of Donor Cells: Karyotyping Methods
Amélie Bonnet-Garnier, Anne-Clemence Veillard, Bertrand Bed’Hom, Hélène Hayes, and Janice Britton-Davidian
8. Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
Eiji Mizutani, Sayaka Wakayama, and Teruhiko Wakayama
9. Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos
Claire Boulesteix and Nathalie Beaujean
10. Visualization of Epigenetic Modifications in Preimplantation Embryos
Hiroshi Kimura and Kazuo Yamagata
11. Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
Sebastian T. Balbach and Michele Boiani
12. Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming
Olga Østrup, Hanne S. Pedersen, Hanne M. Holm, and Poul Hyttel
13. Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
Claire Chazaud
14. Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR)
Nathalie Peynot, Véronique Duranthon, and Daulat Raheem Khan
15. Studying Bovine Early Embryo Transcriptome by Microarray
Isabelle Dufort, Claude Robert, and Marc-André Sirard
16. Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
Annick Lefèvre and Thierry Blachère
17. Micro Chromatin Immunoprecipitation (ChIP) from Early Mammalian Embryos
John Arne Dahl and Arne Klungland
18. Assessing Reprogramming by Chimera Formation and Tetraploid Complementation
Xin Li, Bao-long Xia, Wei Li, and Qi Zhou
19. Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
Bernd Püschel and Alice Jouneau
20. Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
Hélène Kiefer
