Buch, Englisch, Band 2169, 218 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 445 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2169, 218 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 445 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-0734-3
Verlag: Springer US
This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms.
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Preface…Table of Contents…Contributing Authors…
1. Selective Visualization of Caveolae by TEM using APEX2Alexander Ludwig
2. Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and TissuesEric Seemann, Michael M. Kessels, and Britta Qualmann
3. Analysis of Caveolin in Primary CiliaStine K. Morthorst, Johanne B. Mogensen, Søren T. Christensen, and Lotte B. Pedersen
4. Method for Efficient Observation of Caveolin-1 Plasma Membrane by Microscopy Imaging AnalysisTomoya Yamaguchi, Toyoshi Fujimoto, and Takashi Takahashi
5. Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma MembraneTakashi Hirama and Raibatak Das
6. Spatiotemporal Analysis of Caveolae Dynamics using Total Internal Reflection Fluorescence MicroscopyYosuke Senju and Shiro Suetsugu
7. Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 SwitchAdriana M. Zimnicka, Zhenlong Chen, Peter T. Toth, and Richard D. Minshall
8. GPMVs as a Tool to Study Caveolin-Interacting PartnersJoanna Podkalicka and Cedric M. Blouin
9. Biotin Proximity Labeling to Identify Protein-Protein Interactions for Cavin1Carolina Mendoza-Topaz
10. Investigation of Novel Cavin-1/Suppressor of Cytokine 3 (SOCS3) Interactions by Co-Immunoprecipitation, Peptide Pull-Down and Peptide Array Overlay ApproachesJamie J.L. Williams, George S. Baillie, and Timothy M. Palmer
11. Analysis of Protein and Lipid Interactions using Liposome Co-Sedimentation AssaysElin Larsson, Madlen Hubert, and Richard Lundmark
12. Liposome Binding Assay to Characterize Cavin Family Protein Structure and FunctionsMiriam Stoeber
13. Preparation of Caveolin-1 for NMR Spectroscopy ExperimentsSarah M. Plucinsky, Jeffrey A. Julien, and Kerney J. Glover
14. Tagging and Deleting of Endogenous Caveolar Components using CRISPR/Cas9 TechnologyElena Shvets and Carolina Mendoza-Topaz
15. Pulling of Tethers from the Cell Plasma Membrane using Optical TweezersDarius V. Köster
16. Live Confocal Imaging of Zebrafish Notochord Cells under Mechanical Stress In VivoYe-Wheen Lim, Harriet P. Lo, Thomas E. Hall, and Robert G. Parton
17. Study of Caveolae Dependent Mechanoprotection in Huma Muscle Cells using Micropatterning and Live Cell MicroscopyMelissa Dewulf and Cedric M. Blouin
18. Immunofluorescence-Based Analysis of Caveolin-3 in the Diagnostic Management of Neuromuscular DiseasesAndreas Roos, Denisa Hathazi, and Ulrike Schara
