Buch, Englisch, Band 1640, 297 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 7318 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1640, 297 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 7318 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-7164-0
Verlag: Springer
This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.
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Weitere Infos & Material
1. Plant ARGONAUTEs: Features, Functions and Unknowns
Alberto Carbonell
2. Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants
Guodong Ren, Xiaoyan Wang, and Bin Yu
3. In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Taichiro Iki, Masayuki Ishikawa, and Manabu Yoshikawa
4. In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants
Yukihide Tomari and Hiro–oki Iwakawa
5. Establishment of an In Vivo ARGONAUTE Reporter System in Plants
Károly Fátyol and József Burgyán
6. Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants
Alberto Carbonell<
7. Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
Ivett Baksa and György Szittya8. Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants
Cheng–Guo Duan and Jian–Kang Zhu
9. Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana
Cécile Lecampion, Elodie Lanet, and Christophe Robaglia
10. Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
Marina Martinez–Garciaand Mónica Pradillo
11. Isolation and Characterization of ARGONAUTE Mutants in ChlamydomonasTomohito Yamasaki
12. ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic TransformationMeizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li, and Shanfa Lu
13. Cloning and Characterization of ARGONAUTE Genes in Tomato
Zhiqiang Xian, Fang Yan, and Zhengguo Li
14. Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled ProbesAriel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, and Pablo A. Manavella
15. Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes
Jun Hu and Yingguo Zhu
16. Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA Methylation in Plants
Kai Tang, Cheng–Guo Duan, Huiming Zhang and Jian–Kang Zhu
17. Structural and Functional Characterization of Plant ARGONAUTE MID Domains
Filipp Frank and Bhushan Nagar
18. Identification and Analysis of WG/GW ARGONAUTE–Binding Domains
Andrzej Zielezinski and Wojciech M. Karlowski
19. Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
Joshua T. Trujillo and Rebecca A. Mosher
20. Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Ravi K. Singh and Shree P. Pandey
