Buch, Englisch, Band 913, 432 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1034 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 913, 432 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1034 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61779-985-3
Verlag: Humana Press
Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.
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Weitere Infos & Material
Part I. Microelectrode Techniques
1. Patch-Clamp Protocols to Study Cell Ionic Homeostasis under Saline Conditions
Ana María Velarde-Buendía, René Alberto Enríquez-Figueroa, and Igor Pottosin
2. Studying Plant Salt Tolerance with the Voltage Clamp Technique
Zhong-Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, and Michael R. Blatt
3. Using the Multifunctional Xylem Pprobe for In situ Studies of Plant Water and Ion Relations under Saline Conditions
Lars H. Wegner
4. Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes
Tony Miller and Susan Smith
5. Single-Cell Sampling and Analysis (SiCSA)
Wieland Fricke
6. Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations and Systems
Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, and Jian Sun
7. Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-Invasive Microelectrode Measuring MIFE Technique
Sergey Shabala, Tracey Cuin, Lana Shabala and Ian Newman
Part II. Imaging Techniques
8. Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt
Margaret McCully and Martin Canny
9. Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research
Olga Babourina and Zed Rengel
10. Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress
Anuphon Laohavisit, Renato Colaço, and Julia Davies
11. Infrared Thermography in Plant Phenotyping for Salinity Tolerance
Richard A. James and Xavier R.R. Sirault
12. In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy
Yan-Jie Xie and Wen-Biao Shen
Part III. Biochemical Assays
13. Metabolomics for Salinity Research
Ute Roessner and Diane M. Beckles
14. Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping
Anette Lund and Anja Thoe Fuglsang
15. Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues
Andrés Alberto Rodríguez and Edith L. Taleisnik
16. Quantification of the Antioxidant Activity in Salt-Stressed Tissues
Jelena J. Dragišic Maksimovic and Branka D. Živanovic
17. Quantification of Abscisic Acid, Cytokinin and Auxin Content in Salt-Stressed Plant Tissues
Petre I. Dobrev and Radomira Vankova
Part IV. Molecular Techniques
18. Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Sspecific Responses to Salinity Stress in Arabidopsis and Rice
Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson
19. Transformation Using Controlled cDNA Overexpression System
Gábor Rigó, Csaba Papdi, and László Szabados
20. Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress
Prasad Senadheera1 and Frans J.M. Maathuis
21. Marker-Assisted Selection in Plant Breeding for Salinity Tolerance
M. Ashraf, N. A. Akram, Mehboob-ur-Rahman, and M. R. Foolad
22. Transcriptomics on Small Samples
Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, and Matthew Gilliham
23. Plastid Transformation for Abiotic Stress Tolerance in Plants
K.C. Bansal, A. K. Singh, and S. H. Wani
24. Manipulating Expression of Tonoplast Transporters
Zhigang Li, Man Zhou, Qian Hu, Shane Reighard, Shuangrong Yuan, Ning Yuan, Bekir San, Dayong Li, Haiyan Jia, and Hong Luo
25. Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities
Alonso Rodríguez-Navarro1, Begoña Benito, and Olivier Cagnac
Part V. Other Methods
26. Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions
D.T. Britto and H.J. Kronzucker
27. Trait Dissection of Salinity Tolerance with Plant Phenomics
Bettina Berger, Bas de Regt, and Mark Tester
28. Measuring Soil Salinity
Marcus Hardie and Richard Doyle
