Buch, Englisch, Band 1301, 278 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 7609 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1301, 278 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 7609 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-2598-8
Verlag: Springer
This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Weitere Infos & Material
Part I: Introduction to PFGE
1. Pulsed-field Gel Electrophoresis for Disease Monitoring and Control
John Besser
2. Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network
Benjamin Félix, Sophie Roussel, and Bruno Pot
3. PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies
Marion Dalmasso and Kieran Jordan
4. Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak
Niall De Lappe and Martin Cormican
Part II: PFGE and Pathogenic Bacteria
5. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes
Laura Luque-Sastre, Séamus Fanning, and Edward M. Fox
6. Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species
Qiongqiong Yan and Séamus Fanning
7. Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains
Paul Drean and Edward M. Fox
8. Pulsed-Field Gel Electrophoresis of Staphylococcus aureus
George R. Golding, Jennifer Campbell, Dave Spreitzer, and Linda Chui
9. The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile
Wondwossen A. Gebreyes and Pamela R. Fry
10. Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis
Carolina Lúquez, Lavin A. Joseph, and Susan E. Maslanka
11. Pulsed Field Gel Electrophoresis of Yersinia pestis
Tamara Revazishvili and Judith A. Johnson
12. Pulsed Field Gel Electrophoresis of Group A streptococci
Luca Agostino Vitali, Giovanni Gherardi, and Dezemona Petrelli
13. Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni
Ping Zhou and Omar A. Oyarzabal
14. Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa
Honghua Hu and Jim Manos
15. PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC
Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, and Efrain M. Ribot
16. Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica
Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, and Nicola Pugliese
PART III: PFGE and Non-Pathogenic Bacteria
17. PFGE Protocols to Distinguish Subspecies of Lactococcus lactis
Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, and Paul Ritzenthaler
18. The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus
Tomasz Gosiewski and Monika Brzychczy-Wloch
19. Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides
Victoria Chuat and Marion Dalmasso
20. Pulsed Field Gel Electrophoresis for Bifidobacterium
Esther Jiménez, Marta Gómez, and Laura Moles
21. Pulsed Field Gel Electrophoresis for Dairy Propionibacteria
Victoria Chuat, Rosangela de Freitas, and Marion Dalmasso
