Buch, Englisch, Band 1103, 278 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 6952 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1103, 278 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 6952 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-62703-729-7
Verlag: Humana Press
In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Virologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie
- Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
- Naturwissenschaften Biowissenschaften Molekularbiologie
Weitere Infos & Material
1. Identification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites
Navjot Singh and Joseph T. Wade
2. Screening Inhibitory potential of anti-HIV RT RNA Aptamers
Margaret J. Lange and Donald H. Burke
3. Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA
Robert J. Scarborough, Michel V. Lévesque, Jean-Pierre Perreault, and Anne Gatignol
4. Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells
Xiaohong Jiang, Naresh Sunkara, Sangwei Lu, and Fenyong Liu
5. Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target
James Carter, Pruksa Nawtaisong, Velmurugan Balaraman, and Malcolm J. Fraser Jr.
6. Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells
François D’Anjou, Frédéric Couture, Roxane Desjardins and Robert Day
7. Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment
Seong-Wook Lee and Jin-Sook Jeong
8. Characterization of Hairpin Ribozyme Reactions
Preeti Bajajand Christian Hammann
9. Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal
Amell El Korbi, Jonathan Ouellet, Mohammad Reza Naghdi, and Jonathan Perreault
10. Structure-based Virtual Screening for the Identification of RNA-binding Ligands
Peter Daldrop and Ruth Brenk
11. Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays
Francesco Colizzi, Anne-Marie Lamontagne, Daniel A. Lafontaine, and Giovanni Bussi
12. Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection
Valerie T. Tripp and Douglas D. Young
13. Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs
Danielle Biscardo Pedrolli and Matthias Mack
14. Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells
Casey C. Fowler and Yingfu Li
15. Screening Assays to Identify Artificial glmS Ribozyme Activators
Christina E. Lünse and Günter Mayer
16. Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS)
Nathan J. Baird and Adrian R. Ferré-D'Amaré
17. Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies
Marie F. Soulière and Ronald Micura
18. Cell Internalization SELEX: In Vitro Selection for Molecules that Internalize into Cells
Amy Yan and Matthew Levy
19. DNA Electronic Switches Based on Analyte-Responsive Aptamers
Jason M. Thomas, Hua-Zhong Yu, and Dipankar Sen
