Buch, Englisch, Band 1177, 213 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 6213 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1177, 213 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 6213 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-1033-5
Verlag: Springer US
Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.
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Weitere Infos & Material
1. 1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins
Laurie L. Molday and Robert S. Molday
2. Affinity Purification of Heme-Tagged Proteins
Wesley B. Asher and Kara L. Bren
3. Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag
Dongmei Wang and Jiong Hong
4. Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag
Michael J. Coolbaugh and David W. Wood
5. Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag
Aimee Shen
6. SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1
Dennis Kuo, Minghua Nie, and Albert J. Courey
7. Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag
Danielle Needle and David S. Waugh
8. Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins
Yoshimitsu Takakura, Naomi Oka, and Masako Tsunashima
9. Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2
Silvia C. Locatelli-Hoops and Alexei A. Yeliseev
10. Detection of Protein-Protein Interactions Using Tandem Affinity Purification
Ian Goodfellow and Dalan Bailey
11. An Improved in vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
Francesco Faiola, Arven Saunders, Baoyen Dang, and Jianlong Wang
12. Purification of Recombinant Proteins with a Multifunctional GFP Tag
Takashi Murayama and Takuya Kobayashi
13. Targeted Purification of SnAvi-Tagged Proteins
Ursula Schäffer, Ralf Baumeister, and Ekkehard Schulze
14. An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells
Hanhui Ma, Janel R. McLean, Kathleen L. Gould, and Dannel McCollum
15. Bimolecular Affinity Purification - A Variation of TAP with Multiple Applications
Petro Starokadomskyy and Ezra Burstein
