Mead / Arrowood Cryptosporidium

Methods and Protocols
2. Auflage 2026
ISBN: 978-1-0716-4824-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 405 Seiten

Reihe: Springer Protocols

This second edition volume expands on the previous edition with new chapters discussing the latest protocols that encompass the detection, characterization, and genomic analysis of The first few chapters in this book focus on diagnostic assays, such as traditional microscopy-based techniques, nucleic acid-based molecular detection, and immunoassay-based methodologies. Several chapters cover whole genome sequencing methodologies, genotyping approaches, and novel techniques such as hybridization bait capture analyses. A significant portion of this book is dedicated to functional genomics and experimental models, with discussions on tools used for genetic manipulation (including CRISPR/cas9), gene silencing, and host-pathogen interactions. The volume concludes with chapters exploring drug discovery and testing, highlighting high-content screening methods, phenotypic assays, and efficacy testing of potential disinfectants and therapeutics. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, is a valuable tool for researchers interested in gaining more insight on foundational and innovative research, which will inspire future advancements in this field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mead, Jan R.

Arrowood, Michael J.

Inhaltsverzeichnis

Diagnostic Assays: Microscopy.- Diagnostic Assays: Molecular Detection and Species Determination by Real-Time PCR-Based Assays.- Detection of Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.- Genomic Characterization ofspp via iNextEra Library Preparation and Hybridization Bait Capture.- Preparation of for Whole Genome Sequencing.- A Real-Time PCR Based Method for the Sequence Determination of and Subtypes During Epidemiological Investigations.-  Genotyping for Epidemiology Tracking.- A Multi-locus Variable Number Tandem Repeat Analysis (MLVA) Scheme for Epidemiological Investigations ofOptimized Genome Assembly Protocol for Evolutionary Genomic Analyses of A Method to Silence Genes in Use of ncRNAs to Study Host Cell-Parasite Interactions.- Genetic Manipulation of Mouse Models for Use in Infection Studies, Quantification of Parasite Burden Using Flow Cytometry, qPCR and Histopathology, and Confocal Imaging of Oocysts.- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics.- Cell Culture Infectivity to Assess Chlorine Disinfection of Oocysts in Water.- High-Content Screening for Drug Discovery.- Phenotypic High-Throughput Screening and Drug Efficacy Test Against the Growth of In Vitro by qRT-PCR.- Establishing TurboID Proximity Labeling and mAID Conditional Knockdown System in In Vitro Culture of spp. Using Stem Cell-Derived Intestinal Epithelial Monolayers.- Two and Three Dimensional Human Intestinal Tissue Models for Infection.- Oocyst Purification Using Discontinuous Gradient Centrifugation.