Proprotein Convertases

2011
ISBN: 978-1-61779-203-8
Verlag: Humana Press

Buch, Englisch, Band 768, 367 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 910 g

Reihe: Methods in Molecular Biology

Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions.  In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway.  Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Seidah, Nabil G.

Mbikay, Majambu

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Part I: Reminiscences

1. On the Discovery of Precursor Processing

            Donald F. Steiner

2. The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity

            Michel Chrétien

Part II: Biochemistry and Cell Biology

3. The Proprotein Convertases, 20 Years Later

            Nabil G. Seidah

4. Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin

            Ujwal Shinde and Gary Thomas

5. The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression

            Lydiane Funkelstein and Vivian Hook

6. In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies

            Ajoy Basak, Andrew Chen, Swapan Majumdar, and Heather Palmer Smith

7. Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries

            Iris Lindberg and Jon R. Appel

8. Analyses of PCSK9 Post-Translational Modifications Using Time-of-Flight Mass Spectrometry

            Thilina Dewpura and Janice Mayne

Part III: Molecular Biology and Genetics

9. The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling

            Philipp Stawowy and Kai Kappert

10. Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells

            Nathalie Scamuffa, Fabien Calvo, and Abdel-Majid Khatib

11. Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5’-Untranslated Region

            Haidy Tadros, Gunther Schmidt, Francine Sirois, and Majambu Mbikay

12. Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease

            YangXin Fu and Mark W. Nachtigal

13. Genetic and Functional Characterization of PCSK1

            Hélène Choquet, Pieter Stijnen, and John W.M. Creemers

Part IV: Experimental Models

14. Prohormone Processing in Zebrafish

            Michael G. Morash, Kelly Soanes, and Younes Anini

15. Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity

            Babykumari P. Chitramuthu and Hugh P.J. Bennett

16. Characterization of Impaired Processing of Neuropeptides in the Brains of Endoproteases Knockout Mice

            Margery C. Beinfeld

17. Quantitative Peptidomics of Mice Lacking Peptide-Processing Enzymes

            Jonathan Wardman and Lloyd D. Fricker

18. A Proteomic Protocol to Identify Physiological Substrates of Proprotein Convertases

            Guiying Nie and Andrew N. Stephens

19. Neurophenotyping Genetically-Modified Mice for Social Behavior

            Ramona M. Rodriguiz, Jennifer S. Colvin, and William C. Wetsel