Sullivan / Cowan / Eggan Human Embryonic Stem Cells

The Practical Handbook
1. Auflage 2008
ISBN: 978-0-470-51159-6
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 424 Seiten, E-Book

With this valuable practical guide, three members of the HarvardStem Cell Institute have compiled and edited the definite handbookfor the exciting new field of human embryonic stem cell research.The editors have gathered protocols from scientists with extensivereputation and expertise, describing and comparing currently usedtechniques for the culture of human stem cells and discussing thestrengths and weaknesses of the different approaches.
Human Embryonic Stem Cells: The PracticalHandbook contains the first centralised collection ofmethods used in human embryonic stem cell biology. The book coversthe derivation of human stem cell lines, the obtaining of cellsfrom human stem cell banks, the culturing and characterisation ofthe cells, and the differentiation of the cells in vitro andin vivo. Lastly, almost all of these protocols can also beused for analyzing and manipulating induced pluripotency iPS stemcells. This allows an even greater number of opportunities forthose interested in purusing work in pluripotent stem cells,disease modelling, and other aspects of basic regenerative medicineresearch.
The novel and useful focus of this book sets it apart from otheravailable books:
* Compares and evaluates the protocols used in leadinglaboratories working on human embryonic stem cells
* Centred solely on practical protocols for human (not mouse)embryonic stem cell research
* Includes extensive troubleshooting sections
* Addresses the different proclivities and behaviours ofindividual human embryonic cell lines
* Contains techniques currently known only to a small number ofspecialised laboratories worldwide
This handbook represents an essential source of up-to-datepractical information for all cell and developmental biologistsworking with human embryonic stem cells or wishing to enter thefield. It is also essential reading for clinical researchersin areas such as diabetes, cardiovascular disease, and neurologicaldiseases.
Praise from the reviews:
"...a highly readable and useful book... A notablefeature of the book is its air of openness and honesty... Thisbook... will help many to navigate the uncharted waters ofhuman embryonic stem cell biology." BRITISH SOCIETY FOR CELLBIOLOGY
"... the imaginative solutions in this book can inspire us toget past our most frustrating limitations." CELL STEM CELL
"... the richness in the details of each protocol presentedwill certainly encourage more scientists to begin studies of Humanpluripotent stem cells..." REGENERATIVE MEDICINE
"In this fast-moving field, this [handbook] will help driveadvances of more and more researchers." DIFFERENTIATION
"...a valuable resource for seasoned and noviceresearchers... an excellent addition to the reference collection ofany medical library or research laboratory." THE AMERICANMEDICAL ASSOCIATION

Autoren/Hrsg.

Sullivan, Stephen

Cowan, Chad

Eggan, Kevin

Inhaltsverzeichnis

Foreword. Davor Solter
Preface.
Section 1: Obtaining and culturing human embryonic stemcells.
1 Organization and good aseptic technique in the human embryonicstem cell laboratory.
Minal J Patel, Emma L Stephenson, and Stephen L Minger
2 Sourcing human embryonic stem cell lines.
Glyn Stacey
3 Human embryo culture for human embryonic stem cellderivation.
R. Douglas Powers and Jeannine Witmyer
4 Derivation of human embryonic stem cell lines.
Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton,Kevin Eggan and Chad A. Cowan
5 Standard culture of human embryonic stem cells.
Jeremy M. Crook, Rachel Horne, and Alan Colman
6 Culture of human embryonic stem cells in chemically definedconditions.
Julie Hsu Clark and Sheng Ding
Section 2: Characterization of undifferentiated embryonic stemcells.
7 Phenotypic analysis of human embryonic stem cells.
Jonathan S. Draper, Cheryle A. Séguin and Peter W.Andrews
8 Genetic and epigenetic analysis of human embryonic stemcells.
Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova
Section 3: Manipulation of human embryonic stem cells.
9 In vivo differentiation of human embryonic stemcells.
Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou
10 In vitro differentiation of human embryonic stemcells.
A. Henry Sathananthan and Alan Trounson
11 Differentiation of human embryonic stem cells intoextraembryonic cell types.
Cheryle A. Séguin and Jonathan S. Draper
12 Directed differentiation of human embryonic stem cellsinto:
(a) Early endoderm cells.
Kenji Osafune, Alice E. Chen and Douglas A. Melton
(b) Hepatic cells.
Neta Lavon and Nissim Benvenisty
(c) Pancreatic cells.
Hiram Chipperfield
13 Directed differentiation of human embryonic stem cellsinto:
(a) Cardiomyocytes.
Christine Mummery, Robert Passier and Chris Denning
(b) Endothelial cells.
Carrie Soukup, Shulamit Levenberg and Ondine Cleaver
(c) Osteogenic cells.
Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, AliKhademhosseini and Robert Langer
(d) Hematopoietic in vivo repopulating cells.
Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q.Daley
(e) Lymphocytes.
Petter S. Woll and Dan S. Kaufman
(f) Myeloid cells.
Chantal Cerdan and Mickie Bhatia
14 Directed differentiation of human embryonic stem cellsinto:
(a) Forebrain neurons.
Emily A. Davis and Lawrence S.B. Goldstein
(b) Dopaminergic neuron.
Jan Pruszak and Ole Isacson
(c) Spinal motor neurons.
Bao-Yang Hu and Su-Chun Zhang
15 (a) Gene targeting: knock out and knock in byhomologous recombination.
Thomas P. Zwaka
(b) RNA interference in human embryonic stem cells.
M. William Lensch, Asmin Tulpule and Holm Zaehres
(c) Generation of gene reporters using bacterial artificialchromosome recombineering. Andrew J. Washkowitz and David A.Shaywitz
Afterword. Azim Surani
Index.

Über Autor(innen)

Stephen Sullivan: Trinity College, Dublin,Ireland
Kevin Eggan: Harvard University, Cambridge, MA, USA
Chad A. Cowan: Harvard University, Cambridge, MA, USA