Tanimizu | Hepatic Stem Cells | Buch | sack.de

Tanimizu Hepatic Stem Cells



Methods and Protocols

1. Auflage 2019, Band: 1905, 248 Seiten, Gebunden, Book, Format (B × H): 179 mm x 262 mm, Gewicht: 664 g Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-8960-7
Verlag: Springer, Berlin


Tanimizu Hepatic Stem Cells

This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.

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Preface...Table of Contents...Contributing Authors...
Part I Isolation of Progenitor Cells
1.Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor CellsAtsunori Tsuchiya and Shuji Terai
2.Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse LiverNaoki Tanimizu
3.Identification and Isolation of Clonogenic Cholangiocyte in MouseBin Li, Craig Dorrell, Pamela S. Canady, and Leslie Wakefield
4.Isolation and Expansion of Rat Hepatocytic Progenitor Cells   Junichi Kino, Norihisa Ichinohe, Masayuki Ishii, and Toshihiro Mitaka
Part II Characterization of Liver Progenitors In Vivo
5.Genetic Lineage Tracing of Biliary Epithelial CellsTeresa Rubio-Tomás, Beatriz Aguilar-Bravo, and Pau Sancho-Bru
6.Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic RecombinationNicola de Prisco, Eleanor Stout, and Joan Fong-Burgada
7.Analysis for the Heterogeneity of Liver Progenitor Cells Kenji Kamimoto
8.Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver RegenerationSungjin Ko and Donghun Shin
Part III Generation of Hepatocytes, Cholangiocytes, and Their Progenitors
9.Conversion of Fibroblasts to Hepatocytes In Vitro Pengyu Huang, Lulu Sun, Ludi Zhang, and Lijian Hui
10.Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo Guangqi Song, Qinggong Yuan, Zhen Dai, Hsin-Chieh Tsay, Xizhong Shen, Michael Ott, and Amar Deep Sharma
11.Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial CellsTakeshi Katsuda and Takahiro Ochiya
12.Induction of Functional Hepatocytes from Human iPSCsTaketomo Kido and Yuta Koui
13.Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, and Hiromi Chikada
Part IV Reconstitution of Liver Tissue Structures
14.Generation of Hepatic Tissue Structures Using Multicellular Spheroid CultureFumiya Tao, Hirotaka Mihara, and Nobuhiko Kojima
15.Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device Ryo Sudo
16.Generation of Hepatic Organoids with Biliary StructuresTakeshi Katsuda, Takahiro Ochiya, and Yasuyuki Sakai
17.Analysis for Remodeling Hepatic Tissue Structures in 3D during RegenerationKota Kaneko
Part V Liver Injury Models
18.Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells Taro Takami, Kenji Tani, Yasuho Taura, and Isao Sakaida
19.A Rodent Model for Cell Transplantation of Hepatic Progenitor CellsSei Kakinuma and Akihide Kamiya
20.Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature HepatocytesMasahiro Yamamoto, Bing Xin, and Yuji Nishikawa
21.Mouse Model for Cholangiocarcinoma from Peribiliary Glands Hayato Nakagawa, Nobumi Suzuki, and Kazuhiko Koike 


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