Ward / Tosh Mouse Cell Culture

Methods and Protocols
2010
ISBN: 978-1-59745-019-5
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 633, 257 Seiten, eBook

Reihe: Methods in Molecular Biology

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In , expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Zielgruppe

Research

Autoren/Hrsg.

Ward, Andrew

Tosh, David

Inhaltsverzeichnis

1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester

2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Alastair S. Garfield

3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells
Janet Smith and Deborah Merrick

4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference
Jamie A. Davies

5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation
Pierre-Marie del Moral and David Warburton

6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh

7. Isolation and Culture of Embryonic Pancreas and Liver
Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh

8. Isolation and Culture of Mouse Satellite Cells
Antonio Musarò and Laura Barberi

9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures
Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton

10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
Reto Caldelari and Eliane J. Müller

11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells
Matthew J. Smalley

12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes
Magalie A. Ravier and Guy A. Rutter

13. Isolation and Culture of Adult Mouse Hepatocytes
Wan-ChunLi, Kate L. Ralphs, and David Tosh

14. Isolation and Culture of Mouse Intestinal Cells
F. Charles Campbell

15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse
Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward

16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry
Carla Sciarretta and Liliana Minichiello

17. Cell Culture of Primary Cerebellar Granule Cells
Dana Krämer and Liliana Minichiello

18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Henrik Ahlenius and Zaal Kokaia